Imperial College London
Alternate

DrLauraMartinez Gili

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Research Fellow
 
 
 
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Contact

 

l.martinez-gili Website

 
 
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Location

 

Queen Elizabeth the Queen Mother Wing (QEQM)St Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Belda:2022:10.1136/gutjnl-2021-325753,
author = {Belda, E and Voland, L and Tremaroli, V and Falony, G and Adriouch, S and Assmann, KE and Prifiti, E and Aron-Wisnewsky, J and Debedat, J and Le, Roy T and Nielsen, T and Amouyal, C and Andre, S and Andreelli, F and Blueher, M and Chakaroun, R and Chilloux, J and Coelho, LP and Dao, MC and Das, P and Fellahi, S and Forslund, S and Galleron, N and Hansen, TH and Holmes, B and Ji, B and Pedersen, HK and Phuong, L and Le, Chatelier E and Lewinter, C and Manneras-Holm, L and Marquet, F and Myridakis, A and Pelloux, V and Pons, N and Quinquis, B and Rouault, C and Roume, H and Salem, J-E and Sokolovska, N and Sondertoft, NB and Touch, S and Vieira-Silva, S and Galan, P and Holst, J and Gotze, JP and Kober, L and Vestergaard, H and Hansen, T and Hercberg, S and Oppert, J-M and Nielsen, J and Letunic, I and Dumas, M-E and Stumvoll, M and Pedersen, OB and Bork, P and Ehrlich, SD and Zucker, J-D and Baeckhed, F and Raes, J and Clement, K},
doi = {10.1136/gutjnl-2021-325753},
journal = {Gut},
pages = {2463--2480},
title = {Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism},
url = {http://dx.doi.org/10.1136/gutjnl-2021-325753},
volume = {71},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Objectives Gut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear. We examined the human gut microbiome’s functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort. We focused on the role of B vitamins and B7/B8 biotin for regulation of host metabolic state, as these vitamins influence both microbial function and host metabolism and inflammation.Design We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.Results Severe obesity is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes. We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue. In mice, the absence or depletion of gut microbiota by antibiotics confirmed the microbial contribution to host biotin levels. Bariatric surgery, which improves metabolism and inflammation, associates with increased bacterial biotin producers and improved host systemic biotin in humans and mice. Finally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and B vitamins, while limiting weight gain and glycaemic deterioration.Conclusion Strategies combining biotin and prebiotic supplementation could help prevent the deterioration of metabolic states in severe obesity.Trial registration number NCT02059538.
AU  - Belda,E
AU  - Voland,L
AU  - Tremaroli,V
AU  - Falony,G
AU  - Adriouch,S
AU  - Assmann,KE
AU  - Prifiti,E
AU  - Aron-Wisnewsky,J
AU  - Debedat,J
AU  - Le,Roy T
AU  - Nielsen,T
AU  - Amouyal,C
AU  - Andre,S
AU  - Andreelli,F
AU  - Blueher,M
AU  - Chakaroun,R
AU  - Chilloux,J
AU  - Coelho,LP
AU  - Dao,MC
AU  - Das,P
AU  - Fellahi,S
AU  - Forslund,S
AU  - Galleron,N
AU  - Hansen,TH
AU  - Holmes,B
AU  - Ji,B
AU  - Pedersen,HK
AU  - Phuong,L
AU  - Le,Chatelier E
AU  - Lewinter,C
AU  - Manneras-Holm,L
AU  - Marquet,F
AU  - Myridakis,A
AU  - Pelloux,V
AU  - Pons,N
AU  - Quinquis,B
AU  - Rouault,C
AU  - Roume,H
AU  - Salem,J-E
AU  - Sokolovska,N
AU  - Sondertoft,NB
AU  - Touch,S
AU  - Vieira-Silva,S
AU  - Galan,P
AU  - Holst,J
AU  - Gotze,JP
AU  - Kober,L
AU  - Vestergaard,H
AU  - Hansen,T
AU  - Hercberg,S
AU  - Oppert,J-M
AU  - Nielsen,J
AU  - Letunic,I
AU  - Dumas,M-E
AU  - Stumvoll,M
AU  - Pedersen,OB
AU  - Bork,P
AU  - Ehrlich,SD
AU  - Zucker,J-D
AU  - Baeckhed,F
AU  - Raes,J
AU  - Clement,K
DO  - 10.1136/gutjnl-2021-325753
EP  - 2480
PY  - 2022///
SN  - 0017-5749
SP  - 2463
TI  - Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism
T2  - Gut
UR  - http://dx.doi.org/10.1136/gutjnl-2021-325753
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000742089300001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://gut.bmj.com/content/71/12/2463
UR  - http://hdl.handle.net/10044/1/104099
VL  - 71
ER  -
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