Imperial College London
Alternate

DrLauraNolan

Faculty of Medicine, National Heart & Lung Institute

Honorary Research Fellow
 
 
 
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Contact

 

l.nolan

 
 
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Location

 

G45Emmanuel Kaye BuildingRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Nolan:2020:10.1099/mic.0.000956,
author = {Nolan, LM and Turnbull, L and Katrib, M and Osvath, SR and Losa, D and Lazenby, JJ and Whitchurch, CB},
doi = {10.1099/mic.0.000956},
journal = {Microbiology},
pages = {995--1003},
title = {Pseudomonas aeruginosa is capable of natural transformation in biofilms},
url = {http://dx.doi.org/10.1099/mic.0.000956},
volume = {10},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Natural transformation is a mechanism that enables competent bacteria to acquire naked, exogenous DNA from the environment. It is a key process that facilitates the dissemination of antibiotic resistance and virulence determinants throughout bacterial populations. Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen that produces large quantities of extracellular DNA (eDNA) that is required for biofilm formation. P. aeruginosa has a remarkable level of genome plasticity and diversity that suggests a high degree of horizontal gene transfer and recombination but is thought to be incapable of natural transformation. Here we show that P. aeruginosa possesses homologues of all proteins known to be involved in natural transformation in other bacterial species. We found that P. aeruginosa in biofilms is competent for natural transformation of both genomic and plasmid DNA. Furthermore, we demonstrate that type-IV pili (T4P) facilitate but are not absolutely essential for natural transformation in P. aeruginosa.
AU  - Nolan,LM
AU  - Turnbull,L
AU  - Katrib,M
AU  - Osvath,SR
AU  - Losa,D
AU  - Lazenby,JJ
AU  - Whitchurch,CB
DO  - 10.1099/mic.0.000956
EP  - 1003
PY  - 2020///
SN  - 1350-0872
SP  - 995
TI  - Pseudomonas aeruginosa is capable of natural transformation in biofilms
T2  - Microbiology
UR  - http://dx.doi.org/10.1099/mic.0.000956
UR  - https://www.microbiologyresearch.org/docserver/fulltext/micro/166/10/995_micro000956.pdf?expires=1606840026&id=id&accname=guest&checksum=25562305EC18A71A8A5D3F832739D094
UR  - http://hdl.handle.net/10044/1/81706
VL  - 10
ER  -
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