Imperial College London
Alternate

DrLauraNolan

Faculty of Medicine, National Heart & Lung Institute

Honorary Research Fellow
 
 
 
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Contact

 

l.nolan

 
 
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Location

 

G45Emmanuel Kaye BuildingRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Coleman:2011:10.1111/j.1758-2229.2010.00225.x,
author = {Coleman, NV and Yau, S and Wilson, NL and Nolan, LM and Migocki, MD and Ly, M and Crossett, B and Holmes, AJ},
doi = {10.1111/j.1758-2229.2010.00225.x},
journal = {Environmental Microbiology Reports},
pages = {297--307},
title = {Untangling the multiple monooxygenases of <i>Mycobacterium chubuense</i> strain NBB4, a versatile hydrocarbon degrader},
url = {http://dx.doi.org/10.1111/j.1758-2229.2010.00225.x},
volume = {3},
year = {2011}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Summary</jats:title><jats:p><jats:italic>Mycobacterium</jats:italic> strain NBB4 was isolated on ethene as part of a bioprospecting study searching for novel monooxygenase (MO) enzymes of interest to biocatalysis and bioremediation. Previous work indicated that strain NBB4 contained an unprecedented diversity of MO genes, and we hypothesized that each MO type would support growth on a distinct hydrocarbon substrate. Here, we attempted to untangle the relationships between MO types and hydrocarbon substrates. Strain NBB4 was shown to grow on C<jats:sub>2</jats:sub>–C<jats:sub>4</jats:sub> alkenes and C<jats:sub>2</jats:sub>–C<jats:sub>16</jats:sub> alkanes. Complete gene clusters encoding six different monooxygenases were recovered from a fosmid library, including homologues of ethene MO (<jats:italic>etnABCD</jats:italic>), propene MO (<jats:italic>pmoABCD</jats:italic>), propane MO (<jats:italic>smoABCD</jats:italic>), butane MO (<jats:italic>smoXYB1C1Z</jats:italic>), cytochrome P450 (CYP153; <jats:italic>fdxcypfdr</jats:italic>) and alkB (<jats:italic>alkBrubA1rubA2</jats:italic>). Catabolic enzymes involved in ethene assimilation (EtnA, EtnC, EtnD, EtnE) and alkane assimilation (alcohol and aldehyde dehydrogenases) were identified by proteomics, and we showed for the first time that stress response proteins (catalase/peroxidase, chaperonins) were induced by growth on C<jats:sub>2</jats:sub>–C<jats:sub>5</jats:sub> alkanes and ethene. Surprisingly, none of the identified MO genes could be specifically associated with oxidation of small alkanes, and thus the nature of the gaseous alkane MO in NBB4 remains mysterious.</jats:p>
AU  - Coleman,NV
AU  - Yau,S
AU  - Wilson,NL
AU  - Nolan,LM
AU  - Migocki,MD
AU  - Ly,M
AU  - Crossett,B
AU  - Holmes,AJ
DO  - 10.1111/j.1758-2229.2010.00225.x
EP  - 307
PY  - 2011///
SN  - 1758-2229
SP  - 297
TI  - Untangling the multiple monooxygenases of <i>Mycobacterium chubuense</i> strain NBB4, a versatile hydrocarbon degrader
T2  - Environmental Microbiology Reports
UR  - http://dx.doi.org/10.1111/j.1758-2229.2010.00225.x
VL  - 3
ER  -
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