Publications
133 results found
McFarlane C, Girvan P, Branch T, et al., 2016, Efficient lipid peroxidation catalyzed by amyloid-beta-copper complex: observation of chemical oscillation and chaos, 60th Annual Meeting of the Biophysical Society, Publisher: Biophysical Society, Pages: 552A-552A, ISSN: 1542-0086
Branch T, Girvan P, Barahona M, et al., 2015, Kinetics of amyloid-beta/metal ions interactions in the synaptic cleft: experiment and simulation, 10th EBSA European Biophysics Congress, Publisher: Springer Verlag, Pages: S230-S230, ISSN: 0175-7571
Mehta P, Jovanovic G, Ying L, et al., 2015, Is the cellular and molecular machinery docile in the stationary phase of Escherichia coli?, Biochemical Society Transactions, Vol: 43, Pages: 168-171, ISSN: 1470-8752
Branch T, Barahona M, Ying L, 2015, Secondary Metal Binding to Amyloid-Beta Monomer is Insignificant under Synaptic Conditions, 59th Annual Meeting of the Biophysical-Society, Publisher: CELL PRESS, Pages: 385A-385A, ISSN: 0006-3495
Branch T, Girvan P, Barahona M, et al., 2015, Introduction of a Fluorescent Probe to Amyloid-β to Reveal Kinetic Insights into Its Interactions with Copper(II), ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol: 54, Pages: 1227-1230, ISSN: 1433-7851
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- Citations: 40
Jovanovic G, Mehta P, Ying L, et al., 2014, Anionic lipids and the cytoskeletal proteins MreB and RodZ define the spatio-temporal distribution and function of membrane stress controller PspA in <i>Escherichia coli</i>, MICROBIOLOGY-SGM, Vol: 160, Pages: 2374-2386, ISSN: 1350-0872
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- Citations: 15
Zhou W, Vilar R, Ying L, et al., 2014, Transcriptional regulatory roles of G-quadruplex DNA in promoters of genes involved in beta-adrenergic signaling pathway, CARDIOVASCULAR RESEARCH, Vol: 103, ISSN: 0008-6363
Jovanovic G, Mehta P, McDonald C, et al., 2014, The N-Terminal Amphipathic Helices Determine Regulatory and Effector Functions of Phage Shock Protein A (PspA) in <i>Escherichia coli</i>, JOURNAL OF MOLECULAR BIOLOGY, Vol: 426, Pages: 1498-1511, ISSN: 0022-2836
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- Citations: 31
Branch T, Evans M, Barahona M, et al., 2014, Kinetics of Metal Amyloid-Beta Binding and Efficacy of Ligands Targeting Metal Amyloid-Beta Interactions, 58th Annual Meeting of the Biophysical-Society, Publisher: CELL PRESS, Pages: 39A-39A, ISSN: 0006-3495
Branch T, Barahona M, Ying L, 2014, Kinetics of the Interconversion Between Two Physiologically Important Copper-Bound Amyloid-Beta Species, 58th Annual Meeting of the Biophysical-Society, Publisher: CELL PRESS, Pages: 682A-682A, ISSN: 0006-3495
Mehta P, Jovanovic G, Lenn T, et al., 2013, Single molecule imaging of localisations, dynamics and stoichiometry of a regulated enhancer-binding protein in live Escherichia coli cells, 9th European-Biophysical-Societies-Association Congress, Publisher: SPRINGER, Pages: S202-S202, ISSN: 0175-7571
Mehta P, Jovanovic G, Lenn T, et al., 2013, Dynamics and stoichiometry of a regulated enhancer-binding protein in live <i>Escherichia coli</i> cells, NATURE COMMUNICATIONS, Vol: 4, ISSN: 2041-1723
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- Citations: 23
Zhou W, Suntharalingam K, Brand NJ, et al., 2013, Possible Regulatory Roles of Promoter G-Quadruplexes in Cardiac Function-Related Genes - Human TnIc as a Model, PLOS One, Vol: 8, ISSN: 1932-6203
G-quadruplexes (G4s) are four-stranded DNA secondary structures, which are involved in a diverse range of biological processes. Although the anti-cancer potential of G4s in oncogene promoters has been thoroughly investigated, the functions of promoter G4s in non-cancer-related genes are not well understood. We have explored the possible regulatory roles of promoter G4s in cardiac function-related genes using both computational and a wide range of experimental approaches. According to our bioinformatics results, it was found that potential G4-forming sequences are particularly enriched in the transcription regulatory regions (TRRs) of cardiac function-related genes. Subsequently, the promoter of human cardiac troponin I (TnIc) was chosen as a model, and G4s found in this region were subjected to biophysical characterisations. The chromosome 19 specific minisatellite G4 sequence (MNSG4) and near transcription start site (TSS) G4 sequence (−80 G4) adopt anti-parallel and parallel structures respectively in 100 mM KCl, with stabilities comparable to those of oncogene G4s. It was also found that TnIc G4s act cooperatively as enhancers in gene expression regulation in HEK293 cells, when stabilised by a synthetic G4-binding ligand. This study provides the first evidence of the biological significance of promoter G4s in cardiac function-related genes. The feasibility of using a single ligand to target multiple G4s in a particular gene has also been discussed.
Maskuniitty M, Leatherbarrow R, Ying L, 2012, Fluorescence measurements of catalysis by foot-and-mouth disease virus 3C protease, 11th International Biorelated Polymer Symposium / 243rd National Spring Meeting of the American-Chemical-Society (ACS), Publisher: AMER CHEMICAL SOC, ISSN: 0065-7727
Zhou W, Suntharalingam K, Brand N, et al., 2012, Possible regulatory roles of promoter G-quadruplexes in cardiac function related genes: a case study of human cTnI, 2nd Congress of the European-Society-of-Cardiology Council on Basic Cardiovascular Science - Frontiers in Cardiovascular Biology, Publisher: OXFORD UNIV PRESS, Pages: S83-S83, ISSN: 0008-6363
Zhou W, Brand NJ, Ying L, 2011, G-Quadruplexes-Novel Mediators of Gene Function, JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH, Vol: 4, Pages: 256-270, ISSN: 1937-5387
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- Citations: 14
Zhou WH, Brand NJ, Ying LM, 2011, G-quadruplexes - novel mediators of gene function, Journal of Cardiovascular Translational Research
Lenn T, Gkekas CN, Bernard L, et al., 2011, Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching, CHEMICAL COMMUNICATIONS, Vol: 47, Pages: 400-402, ISSN: 1359-7345
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- Citations: 20
Yu AC, Liu TT, Luo GB, et al., 2010, Interaction between nitric oxide and lipid-like DDPA LB film investigated with SHG and AFM, CHINESE JOURNAL OF CHEMISTRY, Vol: 18, Pages: 25-28, ISSN: 1001-604X
Zhou W-H, Ying L-M, 2010, Single Molecule Fluorescence Resonance Energy Transfer and Ensemble Biophysical Characterization of a G-quadruplex Formed in the Promoter of Human Myocyte Enhancer Factor 2D, ACTA PHYSICO-CHIMICA SINICA, Vol: 26, Pages: 1099-1106, ISSN: 1000-6818
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- Citations: 1
Zhou W, Ying L, 2010, Single Molecule FRET Characterization of DNA G-Quadruplexes Formed In The Promoter of Human MEF2D and TNNI3 Genes, BIOPHYSICAL JOURNAL, Vol: 98, Pages: 265A-265A, ISSN: 0006-3495
Fegan A, Shirude PS, Ying L, et al., 2010, Ensemble and single molecule FRET analysis of the structure and unfolding kinetics of the c-kit promoter quadruplexes, CHEMICAL COMMUNICATIONS, Vol: 46, Pages: 946-948, ISSN: 1359-7345
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- Citations: 11
Huang F, Ying L, Neuweiler H, et al., 2009, Reply to Campos et al.: Direct observation versus ambiguous kinetics and thermodynamics, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 106, Pages: E140-E140, ISSN: 0027-8424
Huang F, Rajagopalan S, Settanni G, et al., 2009, Multiple conformations of full-length p53 detected with single-molecule fluorescence resonance energy transfer, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 106, Pages: 20758-20763, ISSN: 0027-8424
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- Citations: 84
Huang F, Ying L, Fersht AR, 2009, Direct observation of barrier-limited folding of BBL by single-molecule fluorescence resonance energy transfer, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 106, Pages: 16239-16244, ISSN: 0027-8424
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- Citations: 55
Ying L, 2009, Applications of nanopipettes in bionanotechnology, Meeting on Bionanotechnology II - From Biomolecular Assembly to Applications, Publisher: PORTLAND PRESS LTD, Pages: 702-706, ISSN: 0300-5127
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- Citations: 25
Engl C, Jovanovic G, Lloyd LJ, et al., 2009, In vivo localizations of membrane stress controllers PspA and PspG in Escherichia coli, Molecular Microbiology, Vol: 73, Pages: 382-396, ISSN: 1365-2958
The phage shock protein (Psp) response in Gram-negative bacteria counteracts membrane stress. Transcription of the PspF regulon (pspABCDE and pspG) in Escherichia coli is induced upon stresses that dissipate the proton motive force (pmf). Using GFP fusions we have visualized the subcellular localizations of PspA (a negative regulator and effector of Psp) and PspG (an effector of Psp). It has previously been proposed that PspA evenly coates the inner membrane of the cell. We now demonstrate that instead of uniformly covering the entire cell, PspA (and PspG) is highly organized into what appear to be distinct functional classes (complexes at the cell pole and the lateral cell wall). Real-time observations revealed lateral PspA and PspG complexes are highly mobile, but absent in cells lacking MreB. Without the MreB cytoskeleton, induction of the Psp response is still observed, yet these cells fail to maintain pmf under stress conditions. The two spatial subspecies therefore appear to be dynamically and functionally distinct with the polar clusters being associated with sensory function and the mobile complexes with maintenance of pmf.
Ko CW, Zhou W, Marsh RJ, et al., 2009, Probing Conformational Motion of Serpin by Time-Resolved and Single Molecule Fluorescence, Publisher: CELL PRESS, Pages: 377A-377A, ISSN: 0006-3495
Ko CW, Wei Z, Marsh RJ, et al., 2009, Probing nanosecond motions of plasminogen activator inhibitor-1 by time-resolved fluorescence anisotropy, MOLECULAR BIOSYSTEMS, Vol: 5, Pages: 1025-1031, ISSN: 1742-206X
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- Citations: 10
Piper JD, Li C, Lo C-J, et al., 2008, Characterization and application of controllable local chemical changes produced by reagent delivery from a nanopipet, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol: 130, Pages: 10386-10393, ISSN: 0002-7863
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- Citations: 31
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