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@article{Zhou:2018:10.1038/s41467-018-07324-5,
author = {Zhou, W and Hu, L and Ying, L and Zhao, Z and Chu, PK and Yu, X-F},
doi = {10.1038/s41467-018-07324-5},
journal = {Nature Communications},
title = {A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection},
url = {http://dx.doi.org/10.1038/s41467-018-07324-5},
volume = {9},
year = {2018}
}

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TY  - JOUR
AB  - Although polymerase chain reaction (PCR) is the most widely used method for DNA amplification, the requirement of thermocycling limits its non-laboratory applications. Isothermal DNA amplification techniques are hence valuable for on-site diagnostic applications in place of traditional PCR. Here we describe a true isothermal approach for amplifying and detecting double-stranded DNA based on a CRISPR–Cas9-triggered nicking endonuclease-mediated Strand Displacement Amplification method (namely CRISDA). CRISDA takes advantage of the high sensitivity/specificity and unique conformational rearrangements of CRISPR effectors in recognizing the target DNA. In combination with a peptide nucleic acid (PNA) invasion-mediated endpoint measurement, the method exhibits attomolar sensitivity and single-nucleotide specificity in detection of various DNA targets under a complex sample background. Additionally, by integrating the technique with a Cas9-mediated target enrichment approach, CRISDA exhibits sub-attomolar sensitivity. In summary, CRISDA is a powerful isothermal tool for ultrasensitive and specific detection of nucleic acids in point-of-care diagnostics and field analyses.
AU  - Zhou,W
AU  - Hu,L
AU  - Ying,L
AU  - Zhao,Z
AU  - Chu,PK
AU  - Yu,X-F
DO  - 10.1038/s41467-018-07324-5
PY  - 2018///
SN  - 2041-1723
TI  - A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-018-07324-5
UR  - http://hdl.handle.net/10044/1/65333
VL  - 9
ER  -

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