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@article{Zhou:2018:10.1002/ange.201806941,
author = {Zhou, W and Cui, H and Ying, L and Yu, X},
doi = {10.1002/ange.201806941},
journal = {Angewandte Chemie},
pages = {10425--10429},
title = {Enhanced Cytosolic Delivery and Release of CRISPR/Cas9 by Black Phosphorus Nanosheets for Genome Editing},
url = {http://dx.doi.org/10.1002/ange.201806941},
volume = {130},
year = {2018}
}

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TY  - JOUR
AB  - <jats:title>Abstract</jats:title><jats:p>A biodegradable twodimensional (2D) delivery platform based on loading black phosphorus nanosheets (BPs) with Cas9 ribonucleoprotein engineered with three nuclear localization signals (NLSs) at C terminus (Cas9N3) is successfully established. The Cas9N3BPs enter cells effectively via membrane penetration and endocytosis pathways, followed by a BPs biodegradationassociated endosomal escape and cytosolic releases of the loaded Cas9N3 complexes. The Cas9N3BPs thus provide efficient genome editing and gene silencing invitro and invivo at a relatively low dose as compared with other nanoparticlebased delivery platforms. This biodegradable 2D delivery platform offers a versatile cytosolic delivery approach for CRISPR/Cas9 ribonucleoprotein and other bioactive macromolecules for biomedical applications.</jats:p>
AU  - Zhou,W
AU  - Cui,H
AU  - Ying,L
AU  - Yu,X
DO  - 10.1002/ange.201806941
EP  - 10429
PY  - 2018///
SN  - 0044-8249
SP  - 10425
TI  - Enhanced Cytosolic Delivery and Release of CRISPR/Cas9 by Black Phosphorus Nanosheets for Genome Editing
T2  - Angewandte Chemie
UR  - http://dx.doi.org/10.1002/ange.201806941
VL  - 130
ER  -

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