Imperial College London
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Dr Laura Martin-Sancho

Faculty of Medicine, Department of Infectious Disease

Lecturer in Molecular Virology
 
 
 
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Contact

 

+44 (0)20 7594 9032 ext 49032laura.martin-sancho Website

 
 
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Location

 

315Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Du:2020:10.1038/s41467-020-16140-9,
author = {Du, Y and Hultquist, JF and Zhou, Q and Olson, A and Tseng, Y and Zhang, T-H and Hong, M and Tang, K and Chen, L and Meng, X and McGregor, MJ and Dai, L and Gong, D and Martin-Sancho, L and Chanda, S and Li, X and Bensenger, S and Krogan, NJ and Sun, R},
doi = {10.1038/s41467-020-16140-9},
journal = {Nature Communications},
title = {mRNA display with library of even-distribution reveals cellular interactors of influenza virus NS1},
url = {http://dx.doi.org/10.1038/s41467-020-16140-9},
volume = {11},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Abstract</jats:title><jats:p>A comprehensive examination of protein-protein interactions (PPIs) is fundamental for the understanding of cellular machineries. However, limitations in current methodologies often prevent the detection of PPIs with low abundance proteins. To overcome this challenge, we develop a mRNA display with library of even-distribution (md-LED) method that facilitates the detection of low abundance binders with high specificity and sensitivity. As a proof-of-principle, we apply md-LED to IAV NS1 protein. Complementary to AP-MS, md-LED enables us to validate previously described PPIs as well as to identify novel NS1 interactors. We show that interacting with FASN allows NS1 to directly regulate the synthesis of cellular fatty acids. We also use md-LED to identify a mutant of NS1, D92Y, results in a loss of interaction with CPSF1. The use of high-throughput sequencing as the readout for md-LED enables sensitive quantification of interactions, ultimately enabling massively parallel experimentation for the investigation of PPIs.</jats:p>
AU  - Du,Y
AU  - Hultquist,JF
AU  - Zhou,Q
AU  - Olson,A
AU  - Tseng,Y
AU  - Zhang,T-H
AU  - Hong,M
AU  - Tang,K
AU  - Chen,L
AU  - Meng,X
AU  - McGregor,MJ
AU  - Dai,L
AU  - Gong,D
AU  - Martin-Sancho,L
AU  - Chanda,S
AU  - Li,X
AU  - Bensenger,S
AU  - Krogan,NJ
AU  - Sun,R
DO  - 10.1038/s41467-020-16140-9
PY  - 2020///
TI  - mRNA display with library of even-distribution reveals cellular interactors of influenza virus NS1
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-020-16140-9
VL  - 11
ER  -
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