Publications
165 results found
Thorasin T, Hoyles L, McCartney AL, 2015, Dynamics and diversity of the ‘Atopobium cluster’ in the human faecal microbiota, and phenotypic characterization of ‘Atopobium cluster’ isolates, Microbiology, Vol: 161, Pages: 565-579, ISSN: 0026-2617
This study monitored the dynamics and diversity of the human faecal ‘Atopobium cluster’ over a 3-month period using a polyphasic approach. Fresh faecal samples were collected fortnightly from 13 healthy donors (six males and seven females) aged between 26 and 61 years. FISH was used to enumerate total (EUB338mix) and ‘Atopobium cluster’ (ATO291) bacteria, with counts ranging between 1.12×1011 and 9.95×1011, and 1.03×109 and 1.16×1011 cells (g dry weight faeces)−1, respectively. The ‘Atopobium cluster’ population represented 0.2–22 % of the total bacteria, with proportions donor-dependent. Denaturing gradient gel electrophoresis (DGGE) using ‘Atopobium cluster’-specific primers demonstrated faecal populations of these bacteria were relatively stable, with bands identified as Collinsella aerofaciens, Collinsella intestinalis/Collinsella stercoris, Collinsella tanakaei, Coriobacteriaceae sp. PEAV3-3, Eggerthella lenta, Gordonibacter pamelaeae, Olsenella profusa, Olsenella uli and Paraeggerthella hongkongensis in the DGGE profiles of individuals. Colony PCR was used to identify ‘Atopobium cluster’ bacteria isolated from faeces (n = 224 isolates). 16S rRNA gene sequence analysis of isolates demonstrated Collinsella aerofaciens represented the predominant (88 % of isolates) member of the ‘Atopobium cluster’ found in human faeces, being found in nine individuals. Eggerthella lenta was identified in three individuals (3.6 % of isolates). Isolates of Collinsella tanakaei, an ‘Enorma’ sp. and representatives of novel species belonging to the ‘Atopobium cluster’ were also identified in the study. Phenotypic characterization of the isolates demonstrated their highly saccharolytic nature and heterogeneous phenotypic profiles, and 97 % of the isolates displayed lipase activity.
Hoyles L, McCartney AL, Neve H, et al., 2014, Characterization of virus-like particles associated with the human faecal and caecal microbiota, Research in Microbiology, ISSN: 1769-7123
This work represents an investigation into the presence, abundance and diversity of virus-like particles (VLPs) associated with human faecal and caecal samples. Various methodologies for the recovery of VLPs from faeces were tested and optimized, including successful down-stream processing of such samples for the purpose of an in-depth electron microscopic analysis, pulsed-field gel electrophoresis and efficient DNA recovery. The applicability of the developed VLP characterization method beyond the use of faecal samples was then verified using samples obtained from human caecal fluid.
Dumas ME, Hoyles L, Chilloux J, et al., 2014, Gut Microbial Metabolomic Predictors of Dietary-induced Obesity and Diabetes, Publisher: AMER DIABETES ASSOC, Pages: A497-A497, ISSN: 0012-1797
Hoyles L, 2014, Facklamia, Lactic Acid Bacteria: Biodiversity and Taxonomy, Editors: Holzapfel, Wood, Publisher: Wiley-Blackwell, ISBN: 9781444333831
Hoyles L, 2014, The genus <i>Facklamia</i>, LACTIC ACID BACTERIA: BIODIVERSITY AND TAXONOMY, Editors: Holzapfel, Wood, Publisher: BLACKWELL SCIENCE PUBL, Pages: 91-98, ISBN: 978-1-4443-3383-1
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- Citations: 6
Inness VL, Khoo C, Gross KL, et al., 2013, An assessment of the prebiotic potential of single and blended substrates in anaerobic in vitro batch culture fermentations using canine faecal samples as inocula., Int J Probiotics Prebiotics, Vol: 8, Pages: 133-144
Previously, using an in vitro static batch culture system, it was found that rice bran (RB), inulin, fibersol, mannanoligosaccharides (MOS), larch arabinogalactan and citrus pectin elicited prebiotic effects (in terms of increased numbers of bifidobacteria and lactic acid bacteria) on the faecal microbiota of a dog. The aim of the present study was to confirm the prebiotic potential of each individual substrate using multiple faecal donors, as well as assessing the prebiotic potential of 15 substrate blends made from them. Anaerobic static and stirred, pH-controlled batch culture systems inoculated with faecal samples from healthy dogs were used for this purpose. Fluorescence in situ hybridization (FISH) analysis using seven oligonucleotide probes targeting selected bacterial groups and DAPI (total bacteria) was used to monitor bacterial populations during fermentation runs. High-performance liquid chromatography was used to measure butyrate produced as a result of bacterial fermentation of the substrates. RB and a MOS/RB blend (1:1, w/w) were shown to elicit prebiotic and butyrogenic effects on the canine microbiota in static batch culture fermentations. Further testing of these substrates in stirred, pH-controlled batch culture fermentation systems confirmed the prebiotic and butyrogenic effects of MOS/RB, with no enhancement of Clostridium clusters I and II and Escherichia coli populations.
Hoyles L, Ortman K, Cardew S, et al., 2013, <i>Corynebacterium</i> <i>uterequi</i> sp nov., a non-lipophilic bacterium isolated from urogenital samples from horses, VETERINARY MICROBIOLOGY, Vol: 165, Pages: 469-474, ISSN: 0378-1135
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- Citations: 9
Hoyles L, Ortman K, Cardew S, et al., 2013, Corynebacterium uterequi sp. nov., a non-lipophilic bacterium isolated from urogenital samples from horses, Veterinary Microbiology, Vol: 165, Pages: 469-474, ISSN: 0378-1135
Three strains of a Gram-positive, catalase-positive, fermentative, non-lipophilic, previously unknown bacterium were isolated from urogenital samples taken from mares in Scotland (M401624/00/1) and Sweden (VM 2074 and VM 2298T). All were deposited with the CCUG with tentative identifications as Corynebacterium spp. The strains were characterized using a polyphasic taxonomic approach. Biochemically, the strains were very similar to each other, but phylogenetically distinct from Corynebacterium species with validly published names (≤95% sequence similarity). rpoB gene sequence data confirmed the strains belonged to the same species (>99% sequence similarity) and were distinct from species with validly published names (>13% sequence divergence). On the basis of phenotypic and sequence data, the strains represent a novel species within the genus Corynebacterium, for which the name Corynebacterium uterequi is proposed. The type strain is VM 2298T (=CCUG 61235T=DSM 45634T), isolated from equine uterus. © 2013 Elsevier B.V.
Murphy J, Royer B, Mahony J, et al., 2013, Biodiversity of lactococcal bacteriophages isolated from 3 Gouda-type cheese-producing plants, JOURNAL OF DAIRY SCIENCE, Vol: 96, Pages: 4945-4957, ISSN: 0022-0302
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- Citations: 38
Hoyles L, Clear JA, McCartney AL, 2013, Use of denaturing gradient gel electrophoresis to detect <i>Actinobacteria</i> associated with the human faecal microbiota, ANAEROBE, Vol: 22, Pages: 90-96, ISSN: 1075-9964
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- Citations: 11
Russell WR, Hoyles L, Flint HJ, et al., 2013, Colonic bacterial metabolites and human health, Current Opinion in Microbiology
The influence of the microbial–mammalian metabolic axis is becoming increasingly important for human health. Bacterial fermentation of carbohydrates (CHOs) and proteins produces short-chain fatty acids (SCFA) and a range of other metabolites including those from aromatic amino acid (AAA) fermentation. SCFA influence host health as energy sources and via multiple signalling mechanisms. Bacterial transformation of fibre-related phytochemicals is associated with a reduced incidence of several chronic diseases. The ‘gut–liver axis’ is an emerging area of study. Microbial deconjugation of xenobiotics and release of aromatic moieties into the colon can have a wide range of physiological consequences. In addition, the role of the gut microbiota in choline deficiency in non-alcoholic fatty liver disease (NAFLD) and insulin resistance is receiving increased attention.
Lasker B, Bell M, Klenk HP, et al., 2013, Kroppenstedtia sanguinis sp. nov., and Kroppenstedtia pulmonis sp. nov., thermoactinomycetes isolated from human clinical sources, International Conference on the Biology of Nocardiae
Thorasin T, Pranteda-Jimenez M, Hoyles L, et al., 2013, Coriobacteriaceae: could gut bacteria be implicated in lipid metabolism?, Gut Microbiota for Health, 2nd World Summit
Jimenez-Pranteda ML, Aguilera M, McCartney AL, et al., 2012, Investigation of the impact of feeding Lactobacillus plantarum CRL 1815 encapsulated in microbially derived polymers on the rat faecal microbiota, JOURNAL OF APPLIED MICROBIOLOGY, Vol: 113, Pages: 399-410, ISSN: 1364-5072
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- Citations: 5
Foster G, Wragg P, Koylass MS, et al., 2012, Isolation of <i>Actinomyces hyovaginalis</i> from sheep and comparison with isolates obtained from pigs, VETERINARY MICROBIOLOGY, Vol: 157, Pages: 471-475, ISSN: 0378-1135
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- Citations: 5
Thorasin T, Hoyles L, McCartney AL, 2012, Investigations of the human faecal Coriobacteriaceae population, Exploring the Human Host–Microbiome Interactions in Health and Disease
Hoyles L, Honda H, Logan NA, et al., 2012, Recognition of greater diversity of <i>Bacillus</i> species and related bacteria in human faeces, RESEARCH IN MICROBIOLOGY, Vol: 163, Pages: 3-13, ISSN: 0923-2508
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- Citations: 34
Hoyles L, McCartney AL, 2012, Genus IV. Mobiluncus., Bergey’s Manual® of Systematic Bacteriology, Editors: Whitman, Goodfellow, Kämpfer, Busse, Trujillo, Suzuki, Ludwig, Publisher: Springer, Pages: 126-139, ISBN: 9780387682334
Includes a revised taxonomic outline for the Actinobacteria or the high G+C Gram positives is based upon the SILVA project as well as a description of greater than 200 genera in 49 families.
Thorasin T, Hoyles L, McCartney AL, 2011, Pilot study investigating the Coriobacteriaceae population of the human gastrointestinal microbiota, International Scientific Conference on Nutraceuticals and Functional Foods – FF2011
Hoyles L, McCartney AL, van Sinderen D, 2011, Isolation of a Klebsiella bacterium–bacteriophage combination from human caecum effluent, SGM Autumn Conference
Ogue-Bon E, Khoo C, Hoyles L, et al., 2011, <i>In vitro</i> fermentation of rice bran combined with <i>Lactobacillus acidophilus</i> 14 150B or <i>Bifidobacterium longum</i> 05 by the canine faecal microbiota, FEMS MICROBIOLOGY ECOLOGY, Vol: 75, Pages: 365-376, ISSN: 0168-6496
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- Citations: 14
Honda H, Hoyles L, Gibson GR, et al., 2011, Impact of GanedenBC30 (Bacillus coagulans GBI-30, 6086) on population dynamics of the human gut microbiota in a continuous culture fermentation system, Int J Probiotics Prebiotics, Vol: 6, Pages: 65-72
Hoyles L, Neve H, McCartney AL, et al., 2010, Investigation of bacteriophage diversity associated with the human gastrointestinal microbiota, Globe Forum Dublin 2010
Roger LC, Costabile A, Holland DT, et al., 2010, Examination of faecal <i>Bifidobacterium</i> populations in breast- and formula-fed infants during the first 18 months of life, MICROBIOLOGY-SGM, Vol: 156, Pages: 3329-3341, ISSN: 1350-0872
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- Citations: 210
Inness VL, Khoo C, Gross KL, et al., 2010, Use of static batch culture systems to investigate the fermentation effects of selected oligosaccharides and fibres by the canine faecal microbiota, Int J Probiotics Prebiotics, Vol: 6, Pages: 57-64
Roberfroid M, Gibson GR, Hoyles L, et al., 2010, Prebiotic effects: metabolic and health benefits, BRITISH JOURNAL OF NUTRITION, Vol: 104, Pages: S1-S63, ISSN: 0007-1145
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- Citations: 1376
Hoyles L, Wallace RJ, 2010, Gastrointestinal tract: fat metabolism in the colon, Handbook of Hydrocarbon and Lipid Microbiology, Editors: McGenity, Meer, Lorenzo, Publisher: Springer, Pages: 3111-3118, ISBN: 9783540775881
Hoyles L, 2010, Gastrointestinal tract: intestinal fatty acid metabolism and implications for health, Handbook of Hydrocarbon and Lipid Microbiology, Editors: McGenity, Meer, Lorenzo, Publisher: Springer, Pages: 3119-3132, ISBN: 9783540775881
Roger LC, Costabile A, Hoyles L, et al., 2010, Examination of faecal Bifidobacterium populations in breast- and formula-fed infants during the first 18 months of life, Rowett–INRA 2010: Gut Microbiology: New Insights into Gut Microbial Ecosystems
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