Imperial College London
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Professor Lesley Hoyles

Faculty of Medicine, Department of Surgery & Cancer

Visiting Professor
 
 
 
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Contact

 

lesley.hoyles11 Website

 
 
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Location

 

Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{DAmato:2019:10.1101/866459,
author = {DAmato, A and Di, Cesare-Mannelli L and Lucarini, E and Man, AL and Le, Gall G and Branca, JJV and Ghelardini, C and Amedei, A and Bertelli, E and Regoli, M and Pacini, A and Luciani, G and Gallina, P and Altera, A and Narbad, A and Gulisano, M and Hoyles, L and Vauzour, D and Nicoletti, C},
doi = {10.1101/866459},
title = {Faecal microbiota transplant from aged donor mice affects spatial learning and memory via modulating hippocampal synaptic plasticity- and neurotransmission-related proteins in young recipients},
url = {http://dx.doi.org/10.1101/866459},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - <jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>The gut-brain axis and the intestinal microbiota are emerging as key players in health and disease. Shifts in intestinal microbiota composition affect a variety of systems, however, evidence of their direct impact on cognitive functions is still lacking. We tested whether faecal microbiota transplant (FMT) from aged donor mice into young adult recipients affected the hippocampus, an area of the central nervous system (CNS) known to be affected by the ageing process, and related functions.</jats:p></jats:sec><jats:sec><jats:title>Methods and Findings</jats:title><jats:p>Young adult mice were transplanted with the microbiota from either aged or age-matched donor mice. Following transplantation, characterization of the microbiotas and metabolomics profiles along with a battery of cognitive and behavioural tests were performed. Label-free quantitative proteomics was employed to monitor protein expression in the hippocampus of the recipients. Gut permeability, levels of circulating cytokines and expression of markers of microglia cells were also assessed. FMT from aged donors led to impaired spatial learning and memory in young adult recipients, whereas anxiety, explorative behaviour and locomotor activity remained unaffected. This was paralleled by altered expression of proteins involved in synaptic plasticity and neurotransmission in the hippocampus. Also, a strong reduction of bacteria associated with short-chain fatty acids (SCFAs) production (<jats:italic>Lachnospiraceae, Faecalibaculum</jats:italic>, and <jats:italic>Ruminococcaceae</jats:italic>) and disorders of the CNS (<jats:italic>Prevotellacea</jats:italic>e and <jats:italic>Ruminococcacea</jats:italic>e) was observed. Finally, microglia cells of the hippocampus fimbria, acquired an ageing-like phenotype, w
AU  - DAmato,A
AU  - Di,Cesare-Mannelli L
AU  - Lucarini,E
AU  - Man,AL
AU  - Le,Gall G
AU  - Branca,JJV
AU  - Ghelardini,C
AU  - Amedei,A
AU  - Bertelli,E
AU  - Regoli,M
AU  - Pacini,A
AU  - Luciani,G
AU  - Gallina,P
AU  - Altera,A
AU  - Narbad,A
AU  - Gulisano,M
AU  - Hoyles,L
AU  - Vauzour,D
AU  - Nicoletti,C
DO  - 10.1101/866459
PY  - 2019///
TI  - Faecal microbiota transplant from aged donor mice affects spatial learning and memory via modulating hippocampal synaptic plasticity- and neurotransmission-related proteins in young recipients
UR  - http://dx.doi.org/10.1101/866459
ER  -
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