Imperial College London
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Professor Lesley Hoyles

Faculty of Medicine, Department of Surgery & Cancer

Visiting Professor
 
 
 
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Contact

 

lesley.hoyles11 Website

 
 
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Location

 

Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Shibu:2020:10.1101/2020.07.24.215400,
author = {Shibu, P and McCuaig, F and McCartney, A and Kujawska, M and Hall, L and Hoyles, L},
doi = {10.1101/2020.07.24.215400},
publisher = {bioRxiv},
title = {The kleboxymycin biosynthetic gene cluster is encoded by several species belonging to the Klebsiella oxytoca complex},
url = {http://dx.doi.org/10.1101/2020.07.24.215400},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - As part of ongoing studies with clinically relevant Klebsiella spp., we characterized the genomes of three clinical GES-5-positive strains originally identified as Klebsiella oxytoca. Average nucleotide identity and phylogenetic analyses showed the strains to be Klebsiella michiganensis. In addition to encoding GES-5, the strains encoded SHV-66, a β-lactamase not previously identified in K. michiganensis. The strains further encoded a range of virulence factors and the kleboxymycin biosynthetic gene cluster (BGC), previously only found in K. oxytoca strains and one strain of Klebsiella grimontii. This BGC, associated with antibiotic-associated haemorrhagic colitis, has not previously been reported in K. michiganensis, and this finding led us to carry out a wide-ranging study to determine the prevalence of this BGC in Klebsiella spp. Of 7,170 publicly available Klebsiella genome sequences screened, 88 encoded the kleboxymycin BGC. All BGC-positive strains belonged to the K. oxytoca complex, with strains of four (K. oxytoca, K. pasteurii, K. grimontii, K. michiganensis) of the six species of the complex found to encode the complete BGC. In addition to being found in K. grimontii strains isolated from preterm infants, the BGC was found in K. oxytoca and K. michiganensis metagenome-assembled genomes recovered from neonates. Detection of the kleboxymycin BGC across the K. oxytoca complex may be of clinical relevance and this cluster should be included in databases characterizing virulence factors, in addition to those characterizing BGCs.
AU  - Shibu,P
AU  - McCuaig,F
AU  - McCartney,A
AU  - Kujawska,M
AU  - Hall,L
AU  - Hoyles,L
DO  - 10.1101/2020.07.24.215400
PB  - bioRxiv
PY  - 2020///
TI  - The kleboxymycin biosynthetic gene cluster is encoded by several species belonging to the Klebsiella oxytoca complex
UR  - http://dx.doi.org/10.1101/2020.07.24.215400
UR  - https://www.biorxiv.org/content/10.1101/2020.07.24.215400v1
UR  - http://hdl.handle.net/10044/1/87328
ER  -
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