Imperial College London
Alternate

ProfessorLuisAragon Alcaide

Faculty of Medicine, Institute of Clinical Sciences

Professor of Genetics
 
 
 
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Contact

 

+44 (0)20 3313 8013luis.aragon

 
 
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Location

 

CRB (Clinical Research Building)Hammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bermudez-Lopez:2016:10.1101/gad.278275.116,
author = {Bermudez-Lopez, M and Villoria, MT and Esteras, M and Jarmuz, A and Torres-Rosell, J and Clemente-Blanco, A and Aragon, Alcaide L},
doi = {10.1101/gad.278275.116},
journal = {Genes & Development},
pages = {1339--1356},
title = {Sgs1’s roles in DNA end resection, HJdissolution, and crossover suppressionrequire a two-step SUMO regulationdependent on Smc5/6},
url = {http://dx.doi.org/10.1101/gad.278275.116},
volume = {30},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The RecQ helicase Sgs1 plays critical roles during DNA repair by homologous recombination, from end resection to Holliday junction (HJ) dissolution. Sgs1 has both pro- and anti-recombinogenic roles, and therefore its activity must be tightly regulated. However, the controls involved in recruitment and activation of Sgs1 at damaged sites are unknown. Here we show a two-step role for Smc5/6 in recruiting and activating Sgs1 through SUMOylation. First, auto-SUMOylation of Smc5/6 subunits leads to recruitment of Sgs1 as part of the STR (Sgs1–Top3–Rmi1) complex, mediated by two SUMO-interacting motifs (SIMs) on Sgs1 that specifically recognize SUMOylated Smc5/6. Second, Smc5/6-dependent SUMOylation of Sgs1 and Top3 is required for the efficient function of STR. Sgs1 mutants impaired in recognition of SUMOylated Smc5/6 (sgs1-SIMΔ) or SUMO-dead alleles (sgs1-KR) exhibit unprocessed HJs at damaged replication forks, increased crossover frequencies during double-strand break repair, and severe impairment in DNA end resection. Smc5/6 is a key regulator of Sgs1's recombination functions.
AU  - Bermudez-Lopez,M
AU  - Villoria,MT
AU  - Esteras,M
AU  - Jarmuz,A
AU  - Torres-Rosell,J
AU  - Clemente-Blanco,A
AU  - Aragon,Alcaide L
DO  - 10.1101/gad.278275.116
EP  - 1356
PY  - 2016///
SN  - 1549-5477
SP  - 1339
TI  - Sgs1’s roles in DNA end resection, HJdissolution, and crossover suppressionrequire a two-step SUMO regulationdependent on Smc5/6
T2  - Genes & Development
UR  - http://dx.doi.org/10.1101/gad.278275.116
UR  - http://genesdev.cshlp.org/content/30/11/1339
UR  - http://hdl.handle.net/10044/1/34429
VL  - 30
ER  -
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