Imperial College London
Alternate

ProfessorLuisAragon Alcaide

Faculty of Medicine, Institute of Clinical Sciences

Professor of Genetics
 
 
 
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Contact

 

+44 (0)20 3313 8013luis.aragon

 
 
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Location

 

CRB (Clinical Research Building)Hammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sen:2016:10.1016/j.molcel.2016.09.007,
author = {Sen, N and Leonard, J and Torres, R and Garcia-Luis, J and Palou-Marin, G and Aragon, Alcaide L},
doi = {10.1016/j.molcel.2016.09.007},
journal = {Molecular Cell},
pages = {134--147},
title = {Physical proximity of sister chromatids promotes top2-dependent intertwining},
url = {http://dx.doi.org/10.1016/j.molcel.2016.09.007},
volume = {64},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Sister chromatid intertwines (SCIs), or catenanes, aretopological links between replicated chromatids thatinterfere with chromosome segregation. The forma-tion of SCIs is thought to be a consequence of forkswiveling during DNA replication, and their removalis thought to occur because of the intrinsic featureof type II topoisomerases (Top2) to simplify DNAtopology. Here, we report that SCIs are also formedindependently of DNA replication during G2/M byTop2-dependent concatenation of cohesed chroma-tids due to their physical proximity. We demonstratethat, in contrast to G2/M, Top2 removes SCIs from co-hesedchromatidsattheanaphaseonset.Importantly,SCI removal in anaphase requires condensin and co-incides with the hyperactivation of condensin DNAsupercoiling activity. This is consistent with the long-standing proposal that condensin provides a bias inTop2functiontowarddecatenation.Acomprehensivemodel for the formation and resolution of toxic SCIentanglements on eukaryotic genomes is proposed.
AU  - Sen,N
AU  - Leonard,J
AU  - Torres,R
AU  - Garcia-Luis,J
AU  - Palou-Marin,G
AU  - Aragon,Alcaide L
DO  - 10.1016/j.molcel.2016.09.007
EP  - 147
PY  - 2016///
SN  - 1097-4164
SP  - 134
TI  - Physical proximity of sister chromatids promotes top2-dependent intertwining
T2  - Molecular Cell
UR  - http://dx.doi.org/10.1016/j.molcel.2016.09.007
UR  - http://hdl.handle.net/10044/1/40855
VL  - 64
ER  -
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