Imperial College London
Alternate

ProfessorLuisAragon Alcaide

Faculty of Medicine, Institute of Clinical Sciences

Professor of Genetics
 
 
 
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Contact

 

+44 (0)20 3313 8013luis.aragon

 
 
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Location

 

CRB (Clinical Research Building)Hammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bermúdez-López:2016:10.1007/978-1-4939-6545-8_4,
author = {Bermúdez-López, M and Aragón, L},
doi = {10.1007/978-1-4939-6545-8_4},
journal = {Methods Mol Biol},
pages = {55--64},
title = {Detection of Cohesin SUMOylation In Vivo.},
url = {http://dx.doi.org/10.1007/978-1-4939-6545-8_4},
volume = {1515},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Cohesin is a protein complex with key roles in chromosome biology, from chromatid segregation to DNA repair. Cohesin function is regulated by several posttranslational modifications, including phosphorylation, acetylation, ubiquitylation, and SUMOylation. Recent studies have shown that cohesin SUMOylation is essential for sister chromatid cohesion during normal cell cycle and in response to DNA damage. Posttranslational modification by the small ubiquitin-like modifier (SUMO) is a field in expansion, however, detecting SUMOylation can be challenging because the amount of modified substrates are usually low and de-conjugation during sample preparation often occurs. In this chapter we describe a method that can be adapted to different model organisms, and substrates to detect SUMOylation. We focus on cohesin and show that SUMOylation indeed occurs in most of the subunits of budding yeast cohesin.
AU  - Bermúdez-López,M
AU  - Aragón,L
DO  - 10.1007/978-1-4939-6545-8_4
EP  - 64
PY  - 2016///
SP  - 55
TI  - Detection of Cohesin SUMOylation In Vivo.
T2  - Methods Mol Biol
UR  - http://dx.doi.org/10.1007/978-1-4939-6545-8_4
UR  - https://www.ncbi.nlm.nih.gov/pubmed/27797073
VL  - 1515
ER  -
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