Imperial College London

DR MICHALIS BARKOULAS

Faculty of Natural SciencesDepartment of Life Sciences

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 5227m.barkoulas

 
 
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Location

 

607Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Hintze:2019:10.1101/849174,
author = {Hintze, M and Koneru, SL and Gilbert, SPR and Katsanos, D and Barkoulas, M},
doi = {10.1101/849174},
title = {A cell fate switch in the C. elegans seam cell lineage occurs through modulation of the Wnt asymmetry pathway in response to temperature increase},
url = {http://dx.doi.org/10.1101/849174},
year = {2019}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - <jats:title>Abstract</jats:title><jats:p>Populations often display consistent developmental phenotypes across individuals despite the inevitable biological stochasticity. Nevertheless, developmental robustness has limits and systems can fail upon change in the environment or the genetic background. We use here the seam cells, a population of epidermal stem cells in <jats:italic>Caenorhabditis elegans</jats:italic>, to study the influence of temperature change and genetic variation on cell fate. Seam cell development has mostly been studied so far in the lab reference strain (N2), grown at 20° temperature. We demonstrate that an increase in culture temperature to 25°, introduces variability in the wild-type seam cell lineage with a proportion of animals showing an increase in seam cell number. We map this increase to lineage-specific symmetrisation events of normally asymmetric cell divisions at the final larval stage, leading to the retention of seam cell fate in both daughter cells. Using genetics and single molecule imaging, we demonstrate that this symmetrisation occurs via changes in the Wnt asymmetry pathway, leading to aberrant Wnt target activation in anterior cell daughters. We find that intrinsic differences in the Wnt asymmetry pathway already exist between seam cells at 20° and this may sensitise cells towards a cell fate switch at increased temperature. Finally, we demonstrate that wild isolates of <jats:italic>C. elegans</jats:italic> display variation in seam cell sensitivity to increased culture temperature, although seam cell numbers are comparable when raised at 20°. Our results highlight how temperature can modulate cell fate decisions in an invertebrate model of stem cell patterning.</jats:p>
AU - Hintze,M
AU - Koneru,SL
AU - Gilbert,SPR
AU - Katsanos,D
AU - Barkoulas,M
DO - 10.1101/849174
PY - 2019///
TI - A cell fate switch in the C. elegans seam cell lineage occurs through modulation of the Wnt asymmetry pathway in response to temperature increase
UR - http://dx.doi.org/10.1101/849174
ER -