Imperial College London

DR MICHALIS BARKOULAS

Faculty of Natural SciencesDepartment of Life Sciences

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 5227m.barkoulas

 
 
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Location

 

607Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Grimbert:2016:10.1016/j.ydbio.2016.05.036,
author = {Grimbert, S and Tietze, K and Barkoulas, M and Sternberg, PW and Félix, MA and Braendle, C},
doi = {10.1016/j.ydbio.2016.05.036},
journal = {Developmental Biology},
pages = {123--135},
title = {Anchor cell signaling and vulval precursor cell positioning establish a reproducible spatial context during C. elegans vulval induction},
url = {http://dx.doi.org/10.1016/j.ydbio.2016.05.036},
volume = {416},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - How cells coordinate their spatial positioning through intercellular signaling events is poorly understood. Here we address this topic using Caenorhabditis elegans vulval patterning during which hypodermal vulval precursor cells (VPCs) adopt distinct cell fates determined by their relative positions to the gonadal anchor cell (AC). LIN-3/EGF signaling by the AC induces the central VPC, P6.p, to adopt a 1° vulval fate. Exact alignment of AC and VPCs is thus critical for correct fate patterning, yet, as we show here, the initial AC-VPC positioning is both highly variable and asymmetric among individuals, with AC and P6.p only becoming aligned at the early L3 stage. Cell ablations and mutant analysis indicate that VPCs, most prominently 1° cells, move towards the AC. We identify AC-released LIN-3/EGF as a major attractive signal, which therefore plays a dual role in vulval patterning (cell alignment and fate induction). Additionally, compromising Wnt pathway components also induces AC-VPC alignment errors, with loss of posterior Wnt signaling increasing stochastic vulval centering on P5.p. Our results illustrate how intercellular signaling reduces initial spatial variability in cell positioning to generate reproducible interactions across tissues.
AU - Grimbert,S
AU - Tietze,K
AU - Barkoulas,M
AU - Sternberg,PW
AU - Félix,MA
AU - Braendle,C
DO - 10.1016/j.ydbio.2016.05.036
EP - 135
PY - 2016///
SN - 1095-564X
SP - 123
TI - Anchor cell signaling and vulval precursor cell positioning establish a reproducible spatial context during C. elegans vulval induction
T2 - Developmental Biology
UR - http://dx.doi.org/10.1016/j.ydbio.2016.05.036
UR - http://hdl.handle.net/10044/1/45299
VL - 416
ER -