Imperial College London
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DR MICHALIS BARKOULAS

Faculty of Natural Sciences, Department of Life Sciences

Reader in Molecular Genetics
 
 
 
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Contact

 

+44 (0)20 7594 5227m.barkoulas

 
 
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Location

 

506Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Katsanos:2021:10.1242/dev.199452,
author = {Katsanos, D and Ferrando-Marco, M and Razzaq, I and Aughey, G and Southall, T and Barkoulas, M},
doi = {10.1242/dev.199452},
journal = {Development},
pages = {1--16},
title = {Gene expression profiling of epidermal cell types in C. elegans using Targeted-DamID.},
url = {http://dx.doi.org/10.1242/dev.199452},
volume = {148},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The epidermis of Caenorhabditis elegans is an essential tissue for survival as it contributes to the formation of the cuticle barrier, as well as facilitates developmental progression and animal growth. Most of the epidermis consists of the hyp7 hypodermal syncytium, the nuclei of which are largely generated by the seam cells that exhibit stem cell-like behaviour during development. How the seam cell progenitors differ transcriptionally from the differentiated hypodermis is poorly understood. Here, we introduce Targeted DamID (TaDa) in C. elegans as a method for identifying genes expressed within a tissue of interest without cell isolation. We show that TaDa signal enrichment profiles can be used to identify genes transcribed in the epidermis and use this method to resolve differences in gene expression between the seam cells and the hypodermis. We finally predict and functionally validate new transcription and chromatin factors acting in seam cell development. These findings provide insights into cell-type-specific gene expression profiles likely associated with epidermal cell fate patterning.
AU  - Katsanos,D
AU  - Ferrando-Marco,M
AU  - Razzaq,I
AU  - Aughey,G
AU  - Southall,T
AU  - Barkoulas,M
DO  - 10.1242/dev.199452
EP  - 16
PY  - 2021///
SP  - 1
TI  - Gene expression profiling of epidermal cell types in C. elegans using Targeted-DamID.
T2  - Development
UR  - http://dx.doi.org/10.1242/dev.199452
UR  - https://www.ncbi.nlm.nih.gov/pubmed/34397094
UR  - https://journals.biologists.com/dev/article/doi/10.1242/dev.199452/271838/Gene-expression-profiling-of-epidermal-cell-types
UR  - http://hdl.handle.net/10044/1/91267
VL  - 148
ER  -
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