Imperial College London
Alternate

ProfessorMarinaBotto

Faculty of Medicine, Department of Immunology and Inflammation

Head of Department, Director of Bioservices
 
 
 
//

Contact

 

+44 (0)20 3313 2316m.botto Website

 
 
//

Assistant

 

Miss Claudia Rocchi +44 (0)20 3313 2315

 
//

Location

 

9N10Commonwealth BuildingHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Wang:2016:10.1371/journal.ppat.1005678,
author = {Wang, Y and Jenkins, SA and Gu, C and Shree, A and Martinez-Moczygemba, M and Herold, J and Botto, M and Wetsel, RA and Xu, Y},
doi = {10.1371/journal.ppat.1005678},
journal = {PLOS Pathogens},
title = {Bacillus anthracis Spore Surface Protein BclA Mediates Complement Factor H Binding to Spores and Promotes Spore Persistence},
url = {http://dx.doi.org/10.1371/journal.ppat.1005678},
volume = {12},
year = {2016}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Spores of Bacillus anthracis, the causative agent of anthrax, are known to persist in the host lungs for prolonged periods of time, however the underlying mechanism is poorly understood. In this study, we demonstrated that BclA, a major surface protein of B. anthracis spores, mediated direct binding of complement factor H (CFH) to spores. The surface bound CFH retained its regulatory cofactor activity resulting in C3 degradation and inhibition of downstream complement activation. By comparing results from wild type C57BL/6 mice and complement deficient mice, we further showed that BclA significantly contributed to spore persistence in the mouse lungs and dampened antibody responses to spores in a complement C3-dependent manner. In addition, prior exposure to BclA deletion spores (ΔbclA) provided significant protection against lethal challenges by B. anthracis, whereas the isogenic parent spores did not, indicating that BclA may also impair protective immunity. These results describe for the first time an immune inhibition mechanism of B. anthracis mediated by BclA and CFH that promotes spore persistence in vivo. The findings also suggested an important role of complement in persistent infections and thus have broad implications.
AU  - Wang,Y
AU  - Jenkins,SA
AU  - Gu,C
AU  - Shree,A
AU  - Martinez-Moczygemba,M
AU  - Herold,J
AU  - Botto,M
AU  - Wetsel,RA
AU  - Xu,Y
DO  - 10.1371/journal.ppat.1005678
PY  - 2016///
SN  - 1553-7366
TI  - Bacillus anthracis Spore Surface Protein BclA Mediates Complement Factor H Binding to Spores and Promotes Spore Persistence
T2  - PLOS Pathogens
UR  - http://dx.doi.org/10.1371/journal.ppat.1005678
UR  - http://hdl.handle.net/10044/1/34546
VL  - 12
ER  -
Download