Imperial College London
Alternate

Professor Martin Buck FRS

Faculty of Natural Sciences, Department of Life Sciences

Senior Research Investigator
 
 
 
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Contact

 

+44 (0)20 7594 5442m.buck

 
 
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Location

 

448Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Schafer:2016:10.1016/j.ab.2016.03.017,
author = {Schafer, J and Jovanovic, G and Kotta-Loizou, I and Buck, M},
doi = {10.1016/j.ab.2016.03.017},
journal = {Analytical Biochemistry},
pages = {56--57},
title = {Single-step method for β-galactosidase assays in Escherichia coli using a 96-well microplate reader},
url = {http://dx.doi.org/10.1016/j.ab.2016.03.017},
volume = {503},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Historically, the lacZ gene is one of the most universally used reporters of gene expression in molecular biology. Its activity can be quantified using an artificial substrate, o-nitrophenyl-ß-d-galactopyranoside (ONPG). However, the traditional method for measuring LacZ activity (first described by J. H. Miller in 1972) can be challenging for a large number of samples, is prone to variability, and involves hazardous compounds for lysis (e.g., chloroform, toluene). Here we describe a single-step assay using a 96-well microplate reader with a proven alternative cell permeabilization method. This modified protocol reduces handling time by 90%.
AU  - Schafer,J
AU  - Jovanovic,G
AU  - Kotta-Loizou,I
AU  - Buck,M
DO  - 10.1016/j.ab.2016.03.017
EP  - 57
PY  - 2016///
SN  - 1096-0309
SP  - 56
TI  - Single-step method for β-galactosidase assays in Escherichia coli using a 96-well microplate reader
T2  - Analytical Biochemistry
UR  - http://dx.doi.org/10.1016/j.ab.2016.03.017
UR  - http://hdl.handle.net/10044/1/32427
VL  - 503
ER  -
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