Imperial College London
Alternate

DrMatthewChild

Faculty of Natural Sciences, Department of Life Sciences

Research Fellow (Sir Henry Dale Fellow)
 
 
 
//

Contact

 

+44 (0)20 7594 5402m.child Website

 
 
//

Location

 

603Sir Alexander Fleming BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Anderson:2020:10.15252/msb.20209475,
author = {Anderson, DP and Benns, HJ and Tate, EW and Child, MA},
doi = {10.15252/msb.20209475},
journal = {Mol Syst Biol},
title = {CRISPR-TAPE: protein-centric CRISPR guide design for targeted proteome engineering.},
url = {http://dx.doi.org/10.15252/msb.20209475},
volume = {16},
year = {2020}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Rational molecular engineering of proteins with CRISPR-based approaches is challenged by the gene-centric nature of gRNA design tools. To address this, we have developed CRISPR-TAPE, a protein-centric gRNA design algorithm that allows users to target specific residues, or amino acid types within proteins. gRNA outputs can be customized to support maximal efficacy of homology-directed repair for engineering purposes, removing time-consuming post hoc curation, simplifying gRNA outputs and reducing CPU times.
AU  - Anderson,DP
AU  - Benns,HJ
AU  - Tate,EW
AU  - Child,MA
DO  - 10.15252/msb.20209475
PY  - 2020///
TI  - CRISPR-TAPE: protein-centric CRISPR guide design for targeted proteome engineering.
T2  - Mol Syst Biol
UR  - http://dx.doi.org/10.15252/msb.20209475
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32485092
VL  - 16
ER  -
Download