Imperial College London
Alternate

DrMatthewChild

Faculty of Natural Sciences, Department of Life Sciences

Research Fellow (Sir Henry Dale Fellow)
 
 
 
//

Contact

 

+44 (0)20 7594 5402m.child Website

 
 
//

Location

 

603Sir Alexander Fleming BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Alves:2021:10.3389/fcimb.2021.728425,
author = {Alves, E and Benns, H and Magnus, L and Dominicus, C and Dobai, T and Blight, J and Wincott, C and Child, M},
doi = {10.3389/fcimb.2021.728425},
journal = {Frontiers in Cellular and Infection Microbiology},
pages = {1--14},
title = {An extracellular redox signal triggers calcium release and impacts the asexual development of Toxoplasma gondii},
url = {http://dx.doi.org/10.3389/fcimb.2021.728425},
volume = {11},
year = {2021}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The ability of an organism to sense and respond to environmental redox fluctuations relies on a signaling network that is incompletely understood in apicomplexan parasites such as Toxoplasma gondii. The impact of changes in redox upon the development of this intracellular parasite is not known. Here, we provide a revised collection of 58 genes containing domains related to canonical antioxidant function, with their encoded proteins widely dispersed throughout different cellular compartments. We demonstrate that addition of exogenous H2O2 to human fibroblasts infected with T.gondii triggers a Ca2+ flux in the cytosol of intracellular parasites that can induce egress. In line with existing models, egress triggered by exogenous H2O2 is reliant upon both Calcium-Dependent Protein Kinase 3 and diacylglycerol kinases. Finally, we show that the overexpression a glutaredoxin-roGFP2 redox sensor fusion protein in the parasitophorous vacuole severely impacts parasite replication. These data highlight the rich redox network that exists in T. gondii, evidencing a link betweenextracellular redox and intracellular Ca2+ signaling that can culminate in parasite egress. Our findings also indicate that the redox potential of the intracellular environment contributes to normal parasitegrowth. Combined, our findings highlight the important role of redox as an unexplored regulator of parasite biology.
AU  - Alves,E
AU  - Benns,H
AU  - Magnus,L
AU  - Dominicus,C
AU  - Dobai,T
AU  - Blight,J
AU  - Wincott,C
AU  - Child,M
DO  - 10.3389/fcimb.2021.728425
EP  - 14
PY  - 2021///
SN  - 2235-2988
SP  - 1
TI  - An extracellular redox signal triggers calcium release and impacts the asexual development of Toxoplasma gondii
T2  - Frontiers in Cellular and Infection Microbiology
UR  - http://dx.doi.org/10.3389/fcimb.2021.728425
UR  - https://www.frontiersin.org/articles/10.3389/fcimb.2021.728425/full
UR  - http://hdl.handle.net/10044/1/90936
VL  - 11
ER  -
Download