Imperial College London
Alternate

DrMatthewChild

Faculty of Natural Sciences, Department of Life Sciences

Research Fellow (Sir Henry Dale Fellow)
 
 
 
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Contact

 

+44 (0)20 7594 5402m.child Website

 
 
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Location

 

603Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kolli:2022:10.1073/pnas.2209729119,
author = {Kolli, SK and Molina-Cruz, A and Araki, T and Geurten, FJA and Ramesar, J and Chevalley-Maurel, S and Kroeze, HJ and Bezemer, S and de, Korne C and Withers, R and Raytselis, N and El, Hebieshy AF and Kim, RQ and Child, MA and Kakuta, S and Hisaeda, H and Kobayashi, H and Annoura, T and Hensbergen, PJ and Franke-Fayard, BM and Barillas-Mury, C and Scheeren, FA and Janse, CJ},
doi = {10.1073/pnas.2209729119},
journal = {Proc Natl Acad Sci U S A},
title = {Malaria parasite evades mosquito immunity by glutaminyl cyclase-mediated posttranslational protein modification.},
url = {http://dx.doi.org/10.1073/pnas.2209729119},
volume = {119},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Glutaminyl cyclase (QC) modifies N-terminal glutamine or glutamic acid residues of target proteins into cyclic pyroglutamic acid (pGlu). Here, we report the biochemical and functional analysis of Plasmodium QC. We show that sporozoites of QC-null mutants of rodent and human malaria parasites are recognized by the mosquito immune system and melanized when they reach the hemocoel. Detailed analyses of rodent malaria QC-null mutants showed that sporozoite numbers in salivary glands are reduced in mosquitoes infected with QC-null or QC catalytically dead mutants. This phenotype can be rescued by genetic complementation or by disrupting mosquito melanization or phagocytosis by hemocytes. Mutation of a single QC-target glutamine of the major sporozoite surface protein (circumsporozoite protein; CSP) of the rodent parasite Plasmodium berghei also results in melanization of sporozoites. These findings indicate that QC-mediated posttranslational modification of surface proteins underlies evasion of killing of sporozoites by the mosquito immune system.
AU  - Kolli,SK
AU  - Molina-Cruz,A
AU  - Araki,T
AU  - Geurten,FJA
AU  - Ramesar,J
AU  - Chevalley-Maurel,S
AU  - Kroeze,HJ
AU  - Bezemer,S
AU  - de,Korne C
AU  - Withers,R
AU  - Raytselis,N
AU  - El,Hebieshy AF
AU  - Kim,RQ
AU  - Child,MA
AU  - Kakuta,S
AU  - Hisaeda,H
AU  - Kobayashi,H
AU  - Annoura,T
AU  - Hensbergen,PJ
AU  - Franke-Fayard,BM
AU  - Barillas-Mury,C
AU  - Scheeren,FA
AU  - Janse,CJ
DO  - 10.1073/pnas.2209729119
PY  - 2022///
TI  - Malaria parasite evades mosquito immunity by glutaminyl cyclase-mediated posttranslational protein modification.
T2  - Proc Natl Acad Sci U S A
UR  - http://dx.doi.org/10.1073/pnas.2209729119
UR  - https://www.ncbi.nlm.nih.gov/pubmed/35994647
VL  - 119
ER  -
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