Publications
226 results found
Cordeiro MF, Reichel MB, D'Esposito F, et al., 1997, Modulating effects of TGF-B I, 2.4 3 and mitomycin-C MMO on conjunctival scarring in the mouse, Investigative Ophthalmology and Visual Science, Vol: 38, ISSN: 0146-0404
Purpose TGF-B is known to be a potent giowth factor involved in wound healing Although TGF-β 1 & 2 are established stimulate rs of the scarring response. TGF-β3 has been advocated as an antiscarring agent TGF-12 is believed to be the more important ocular isoform. Using our recently established mouse model of conjunctival scarring, we investigated the effect of TGF-β 1, 2 & 3 and MMC on 6 week old BALB/c mice Method Mice eyes were randomly allocated to one of 8 treatn ents' subconjunctival injections of either 25ul of TGF-β 1 or 2 or 3 (10-9M), or MMC (0.4 01 0.1 mg/ml) or control (PB S-carrier), or treatment with MMC (0.4 mg/ml) followed by subconjunctival TGF-β2 (10-9M) or control 24 hours later. Mice were assessed clinically and sacrificed (6 eyes/ treatmentAimc point) at f> hours, 1, 3, and 7 days after surgery Enucleated eyi s were analysed for development of scar tissue using H&E and special stains for oxytalai and collagen Immunostaming for inflammatory cell subsets was also performed Results 1 Jl singly-treated TGF-β eyes showed a rapid and exaggerated scarring response compared to ;ontrol, with an earlier onset of fibroblast activity and subconjunctival scar tissue deposition. MMC treatment was characterised by an initial vigorous inflammatory response (in a dcse-related manner) with evidence of decreased fibroblastic activity and reduced scar depo .ition. This response was significantly altered b> TGF-B2 treatment, with histological evidaice of stimulated scarring up to 7 days after treatment. Conclusion TGF-β l, 2 & 3 stimulate as compared to MMC which inhibits the conjunctival scarring response. Application of TGF-B2 reversed the effects of MMC This may be an important clinical finding and suggests that TGF-β is a potent modulator of the conjunctival scarring response, and may le used to modify the anii-scarrmg effects of antimetabolites such as MMC.
Constable PH, Crowston JG, Ocdeston NL, et al., 1997, The effect of but a radiation on the wound healing functions of human Tenon's fibroblasts, Investigative Ophthalmology and Visual Science, Vol: 38, ISSN: 0146-0404
Purpose Beta radiation has been shown to be an effectue adjuvant treatment for preventing bleb failure after trabeculectomy, and avoids many of the complications associated with liquid antiproliferatives. including formation of cystic blebs. Little is known of the underlying biological effects of radiation on human Tenon's fibroblasts (HTF) and their wound healing functions, and this may limit clinical development of this treatment. We have therefore investigated the effects of beta radiation on HTF proliferation, contraction and migration. Methods HTF were grown in tissue culture and irradiated using a Strontium 90 source (0 2000 cGy). Cell proliferation over a 28 day period after treatment was studied, and HTF viability demonstrated using vital dye exclusion. The effect of radiation on cell mediated contraction was investigated using fibroblast populated collagen gels, and cell migration was studied using Costar microporous membranes. Results HTF proliferation was significantly inhibited by beta radiation in a dose dependent manner (p<0.05 at alt time points). Irradiated cells remained viable but growth arrested over this period. Irradiated HTF were unaffected in their ability to migrate or to contract collagen gels. Conclusions Single doses of beta radiation inhibit HTF proliferalion by inducing long term growth arrest of the cells. However. HTF irradiated at these clinically relevant doses are unaffected in their ability to contract or migrate, unlike the effects of 5-FU or MMC treatment. These novel findings may help explain the different morphology that irradiated blebs have compared to blebs treated with liquid antiproliferatives. and will hopefully facilitate development of this potentially extremely useful treatment. Supported by the T.F.C.Frost Trust and the International Glaucoma Association.
Cordeiro MF, Constable PH, Alexander RA, et al., 1997, Effect of varying the mitomycin-C treatment area in glaucoma filtration surgery in the rabbit, INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol: 38, Pages: 1639-1646, ISSN: 0146-0404
- Author Web Link
- Cite
- Citations: 86
Mulholland B, Cordeiro MF, Constable PH, et al., 1997, A new modified model of glaucoma filtration surgery in the rabbit, Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, Pages: 1227-1227, ISSN: 0146-0404
Wilkins MR, Crowston JG, Cordeiro MF, et al., 1997, Antimetabolites, Seminars in Ophthalmology, Vol: 12, Pages: 143-150, ISSN: 0882-0538
Antimetabolites have revolutionized filtration surgery in patients with a high risk of scarring postoperatively. There are many different categories of patients with different healing responses, and these patients may need different degrees of wound modulation. For patients with a high risk of failure due to scarring, intraoperative mitomycin-C (MMC) appears to be superior to 5-fluouracil (5-FU) injections in terms of intraocular pressure (IOP) control, convenience, and corneal side effects. For lower risk patients, weaker intraoperative agents such as 5-FU may be more appropriate. Long-term complications of antimetabolites may include leaking blabs, hypotony with maculopathy, and an increased incidence of endophthalmitis. The rationale behind the choice of antimetabolite and surgical details, including the technique of antimetabolite application, surgical site positioning, conjunctival edge protection, scleral flap, and suture technique are discussed.
Cordeiro MF, Occleston NL, Khaw PT, 1997, New concepts: Manipulation of the wound-healing response, Publisher: KARGER, ISBN: 3-8055-6443-0
- Author Web Link
- Cite
- Citations: 9
Cordeiro MF, Stanford MR, Phillips PM, et al., 1997, Relationship of diabetic microvascular complications to outcome in panretinal photocoagulation treatment of proliferative diabetic retinopathy, EYE, Vol: 11, Pages: 531-536, ISSN: 0950-222X
- Author Web Link
- Cite
- Citations: 12
Cordeiro MF, Occleston NL, Constable PH, et al., 1996, Effect of TGF-β1, 2 and 3 on human ocular fibroblast- mediated collagen lattice contraction and matrix metalloprotease production in lattices and monolayers, Investigative Ophthalmology and Visual Science, Vol: 37, ISSN: 0146-0404
Purpose: Fibroblast-mediated contraction is an important part of the ocular healing response. Although TGF-β1 & β2 have been implicated as promoting scarring, the role of TGF-β3 is controversial. We aimed to determine the effect of the three human isoforms of TGF-β on cell-mediated collagen contraction. In addition, as previous work from this laboratory has shown matrix metalloproteases (MMPs) to be essential for collagen contraction, we studied the effects of TGF-β on MMP production. Methods: Collagen lattices were seeded with human Tenons capsule fibroblasts and fed with serum-free medium containing TGF-β 1, 2 or 3 (10-15M to 10-8M solutions). The effects of each isoform upon collagen contraction were investigated up to 14 days post-treatment. Their effect on MMP production by ocular fibroblasts in collagen lattices and monolayers was compared. Results: Treatment with TGF-β1, β2 and β3 stimulated collagen contraction compared to controls in a concentration-dependent manner, with greatest contraction (90% reduction in surface area compared to controls (p<0.05)) seen at 10-9M. A correlation between the degree of collagen contraction and MMP production was demonstrated. Each TGF-β isoform induced different MMP profiles in contracting gels and monolayers, and peak MMP production was associated with maximal collagen contraction. Conclusion: All isoforms of TGF-β stimulate ocular fibroblast mediated-collagen contraction in a concentration-dependent manner. This is associated with an increase in MMP activity. TGF-β induces different MMP profiles from fibroblasts in collagen lattices as opposed to monolayers.
Constable PH, Occleston NL, Cordeiro MF, et al., 1996, Early growth arrest of human Tenons fibroblasts following single doses of beta irradiation., INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol: 37, Pages: 99-99, ISSN: 0146-0404
Reichel ML, Cordeiro MF, Alexander RA, et al., 1996, A new model of conjunctival scarring in the mouse eye, INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol: 37, Pages: 91-91, ISSN: 0146-0404
- Author Web Link
- Cite
- Citations: 2
Occleston NL, Tarnuzzer RW, Waters L, et al., 1996, Inhibition of matrix metalloproteinases inhibits anterior segment fibroblast mediated collagen contraction, INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol: 37, Pages: 4660-4660, ISSN: 0146-0404
- Author Web Link
- Cite
- Citations: 1
CORDEIRO MF, STANFORD M, SHILLING JS, et al., 1995, ELECTRORETINOGRAM AS INDICATOR OF PROGNOSIS OF CENTRAL RETINAL VEIN OCCLUSION, BRITISH JOURNAL OF OPHTHALMOLOGY, Vol: 79, Pages: 507-507, ISSN: 0007-1161
CORDEIRO MF, LLOYD ME, SPALTON DJ, et al., 1994, ISCHEMIC OPTIC NEUROPATHY, TRANSVERSE MYELITIS, AND EPILEPSY IN PHOSPHOLIPID POSITIVE SYSTEMIC LUPUS-ERYTHEMATOSUS, JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY, Vol: 57, Pages: 1142-1143, ISSN: 0022-3050
- Author Web Link
- Cite
- Citations: 30
CORDEIRO MF, GRAHAM EM, 1994, RETINAL INFECTIONS, BRITISH JOURNAL OF HOSPITAL MEDICINE, Vol: 51, Pages: 402-408, ISSN: 0007-1064
CORDEIRO MF, JOLLY BC, DACRE JE, 1993, THE EFFECT OF FORMAL INSTRUCTION IN OPHTHALMOSCOPY ON MEDICAL-STUDENT PERFORMANCE, MEDICAL TEACHER, Vol: 15, Pages: 321-325, ISSN: 0142-159X
- Author Web Link
- Cite
- Citations: 33
CORDEIRO MF, ARNOLD KG, 1991, DIGOXIN TOXICITY PRESENTING AS DYSPHAGIA AND DYSPHONIA, BRITISH MEDICAL JOURNAL, Vol: 302, Pages: 1025-1025, ISSN: 0959-8138
- Author Web Link
- Cite
- Citations: 3
This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.