Imperial College London
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Dr Michael Crone

Faculty of Medicine, Department of Infectious Disease

Visiting Researcher
 
 
 
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Contact

 

m.crone Website

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Guerra-Assunção:2021:10.1101/2021.04.12.21255084,
author = {Guerra-Assunção, JA and Randell, PA and Boshier, FAT and Crone, MA and Pang, J and Mahungu, T and Freemont, PS and Breuer, J},
doi = {10.1101/2021.04.12.21255084},
title = {Reliability of Spike Gene Target Failure for ascertaining SARS-CoV-2 lineage B.1.1.7 prevalence in a hospital setting},
url = {http://dx.doi.org/10.1101/2021.04.12.21255084},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Abstract</jats:title><jats:p>The appearance of the SARS-CoV-2 lineage B.1.1.7 in the UK in late 2020, associated with faster transmission, sparked the need to find effective ways to monitor its spread. The set of mutations that characterise this lineage include a deletion in position 69 and 70 of the spike protein, which is known to be associated with Spike Gene Target Failure (SGTF) in a commonly used three gene diagnostic qPCR assay. The lower cost and faster turnaround times compared to whole genome sequencing make the use of qPCR for monitoring of the variant spread an attractive proposition. However, there are several potential issues with this approach. Here we use 826 SARS-CoV-2 samples collected in a hospital setting as part of the Hospital Onset COVID Infection (HOCI) study where qPCR was used for viral detection, followed by whole genome sequencing (WGS), to identify the factors to consider when using SGTF to infer lineage B.1.1.7 prevalence in a hospital setting, with potential implications for locations where this variant has recently been introduced.</jats:p>
AU  - Guerra-Assunção,JA
AU  - Randell,PA
AU  - Boshier,FAT
AU  - Crone,MA
AU  - Pang,J
AU  - Mahungu,T
AU  - Freemont,PS
AU  - Breuer,J
DO  - 10.1101/2021.04.12.21255084
PY  - 2021///
TI  - Reliability of Spike Gene Target Failure for ascertaining SARS-CoV-2 lineage B.1.1.7 prevalence in a hospital setting
UR  - http://dx.doi.org/10.1101/2021.04.12.21255084
ER  -
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