Imperial College London
Alternate

Emeritus ProfessorMichaelSchneider

Faculty of Medicine, National Heart & Lung Institute

Emeritus Professor in Cardiology
 
 
 
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Contact

 

+44 (0)013 34621727m.d.schneider Website

 
 
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Location

 

ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Speidel:2017:10.1021/acscentsci.7b00039,
author = {Speidel, AT and Stuckey, DJ and Chow, LW and Jackson, LH and Noseda, M and Abreu, Pavia M and Schneider, MD and Stevens, MM},
doi = {10.1021/acscentsci.7b00039},
journal = {ACS Central Science},
pages = {338--348},
title = {Multi-modal hydrogel-based platform to deliver and monitor cardiac progenitor/stem cell engraftment},
url = {http://dx.doi.org/10.1021/acscentsci.7b00039},
volume = {3},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Retention and survival of transplanted cells are major limitations to the efficacy of regenerative medicine, with short-term paracrine signals being the principal mechanism underlying current cell therapies for heart repair. Consequently, even improvements in short-term durability may have a potential impact on cardiac cell grafting. We have developed a multimodal hydrogel-based platform comprised of a poly(ethylene glycol) network cross-linked with bioactive peptides functionalized with Gd(III) in order to monitor the localization and retention of the hydrogel in vivo by magnetic resonance imaging. In this study, we have tailored the material for cardiac applications through the inclusion of a heparin-binding peptide (HBP) sequence in the cross-linker design and formulated the gel to display mechanical properties resembling those of cardiac tissue. Luciferase-expressing cardiac stem cells (CSC-Luc2) encapsulated within these gels maintained their metabolic activity for up to 14 days in vitro. Encapsulation in the HBP hydrogels improved CSC-Luc2 retention in the mouse myocardium and hind limbs at 3 days by 6.5- and 12- fold, respectively. Thus, this novel heparin-binding based, Gd(III)-tagged hydrogel and CSC-Luc2 platform system demonstrates a tailored, in vivo detectable theranostic cell delivery system that can be implemented to monitor and assess the transplanted material and cell retention.
AU  - Speidel,AT
AU  - Stuckey,DJ
AU  - Chow,LW
AU  - Jackson,LH
AU  - Noseda,M
AU  - Abreu,Pavia M
AU  - Schneider,MD
AU  - Stevens,MM
DO  - 10.1021/acscentsci.7b00039
EP  - 348
PY  - 2017///
SN  - 2374-7951
SP  - 338
TI  - Multi-modal hydrogel-based platform to deliver and monitor cardiac progenitor/stem cell engraftment
T2  - ACS Central Science
UR  - http://dx.doi.org/10.1021/acscentsci.7b00039
UR  - http://hdl.handle.net/10044/1/45710
VL  - 3
ER  -
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