Imperial College London
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DrMarcoDi Antonio

Faculty of Natural Sciences, Department of Chemistry

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 5866m.di-antonio

 
 
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Location

 

207LMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Mao:2018:10.1038/s41594-018-0131-8,
author = {Mao, S-Q and Ghanbarian, AT and Spiegel, J and Cuesta, SM and Beraldi, D and Di, Antonio M and Marsico, G and Hansel-Hertsch, R and Tannahill, D and Balasubramanian, S},
doi = {10.1038/s41594-018-0131-8},
journal = {Nature Structural and Molecular Biology},
pages = {951--957},
title = {DNA G-quadruplex structures mold the DNA methylome},
url = {http://dx.doi.org/10.1038/s41594-018-0131-8},
volume = {25},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Control of DNA methylation level is critical for gene regulation, and the factors that govern hypomethylation at CpG islands (CGIs) are still being uncovered. Here, we provide evidence that G-quadruplex (G4) DNA secondary structures are genomic features that influence methylation at CGIs. We show that the presence of G4 structure is tightly associated with CGI hypomethylation in the human genome. Surprisingly, we find that these G4 sites are enriched for DNA methyltransferase 1 (DNMT1) occupancy, which is consistent with our biophysical observations that DNMT1 exhibits higher binding affinity for G4s as compared to duplex, hemi-methylated, or single-stranded DNA. The biochemical assays also show that the G4 structure itself, rather than sequence, inhibits DNMT1 enzymatic activity. Based on these data, we propose that G4 formation sequesters DNMT1 thereby protecting certain CGIs from methylation and inhibiting local methylation.
AU  - Mao,S-Q
AU  - Ghanbarian,AT
AU  - Spiegel,J
AU  - Cuesta,SM
AU  - Beraldi,D
AU  - Di,Antonio M
AU  - Marsico,G
AU  - Hansel-Hertsch,R
AU  - Tannahill,D
AU  - Balasubramanian,S
DO  - 10.1038/s41594-018-0131-8
EP  - 957
PY  - 2018///
SN  - 1545-9985
SP  - 951
TI  - DNA G-quadruplex structures mold the DNA methylome
T2  - Nature Structural and Molecular Biology
UR  - http://dx.doi.org/10.1038/s41594-018-0131-8
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000446468200007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.nature.com/articles/s41594-018-0131-8
VL  - 25
ER  -
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