Imperial College London
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ProfessorMarcDionne

Faculty of Natural Sciences, Department of Life Sciences

Professor of Innate Immunity
 
 
 
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Contact

 

+44 (0)20 7594 5776m.dionne Website

 
 
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Location

 

G.23Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Rismondo:2021:10.1128/JB.00553-20,
author = {Rismondo, J and Schulz, LM and Yacoub, M and Wadhawan, A and Hoppert, M and Dionne, MS and Gründling, A},
doi = {10.1128/JB.00553-20},
journal = {Journal of Bacteriology},
pages = {1--16},
title = {EslB is required for cell wall biosynthesis and modification in Listeria monocytogenes.},
url = {http://dx.doi.org/10.1128/JB.00553-20},
volume = {203},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Lysozyme is an important component of the innate immune system. It functions by hydrolysing the peptidoglycan (PG) layer of bacteria. The human pathogen Listeria monocytogenes is intrinsically lysozyme resistant. The peptidoglycan N-deacetylase PgdA and O-acetyltransferase OatA are two known factors contributing to its lysozyme resistance. Furthermore, it was shown that the absence of components of an ABC transporter, here referred to as EslABC, leads to reduced lysozyme resistance. How its activity is linked to lysozyme resistance is still unknown. To investigate this further, a strain with a deletion in eslB, coding for a membrane component of the ABC transporter, was constructed in L. monocytogenes strain 10403S. The eslB mutant showed a 40-fold reduction in the minimal inhibitory concentration to lysozyme. Analysis of the PG structure revealed that the eslB mutant produced PG with reduced levels of O-acetylation. Using growth and autolysis assays, we show that the absence of EslB manifests in a growth defect in media containing high concentrations of sugars and increased endogenous cell lysis. A thinner PG layer produced by the eslB mutant under these growth conditions might explain these phenotypes. Furthermore, the eslB mutant had a noticeable cell division defect and formed elongated cells. Microscopy analysis revealed that an early cell division protein still localized in the eslB mutant indicating that a downstream process is perturbed. Based on our results, we hypothesize that EslB affects the biosynthesis and modification of the cell wall in L. monocytogenes and is thus important for the maintenance of cell wall integrity.IMPORTANCE The ABC transporter EslABC is associated with the intrinsic lysozyme resistance of Listeria monocytogenes However, the exact role of the transporter in this process and in the physiology of L. monocytogenes is unknown. Using different assays to characterize an eslB deletion strain, we found that the absence of EslB not only af
AU  - Rismondo,J
AU  - Schulz,LM
AU  - Yacoub,M
AU  - Wadhawan,A
AU  - Hoppert,M
AU  - Dionne,MS
AU  - Gründling,A
DO  - 10.1128/JB.00553-20
EP  - 16
PY  - 2021///
SN  - 0021-9193
SP  - 1
TI  - EslB is required for cell wall biosynthesis and modification in Listeria monocytogenes.
T2  - Journal of Bacteriology
UR  - http://dx.doi.org/10.1128/JB.00553-20
UR  - https://www.ncbi.nlm.nih.gov/pubmed/33229460
UR  - https://jb.asm.org/content/203/4/e00553-20/
UR  - http://hdl.handle.net/10044/1/84613
VL  - 203
ER  -
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