Partial covariance two-dimensional mass spectrometry for bio-molecules
The principle of pC-2DMS:
The inherent scan-to-scan fluctuations in the abundances of fragments born in the same (panel a) and consecutive reactions follow each other in a series of individual MS/MS scans (panel b). pC-2DMS exploits this property to identify the correlating fragment pairs, by analysing a series of repeated MS/MS scans and calculating the partial covariance between all fragment intensities. The correlating fragment pairs are joined by positive islands on the pC-2DMS map (panel c).