Imperial College London
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DrMikeEmerson

Faculty of Medicine, National Heart & Lung Institute

Reader in Platelet Pharmacology
 
 
 
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Contact

 

+44 (0)7941 828 744m.emerson Website

 
 
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Location

 

537ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Taylor:2018:10.12688/f1000research.14456.1,
author = {Taylor, KA and Emerson, M},
doi = {10.12688/f1000research.14456.1},
journal = {F1000Research},
title = {Refinement of a mouse cardiovascular model: development, application and dissemination},
url = {http://dx.doi.org/10.12688/f1000research.14456.1},
volume = {7},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - European and UK legislation requires all animal procedures to be conductedwith consideration to reduction, refinement and replacement. In this review,3Rs developments are discussed in the field of platelet biology andthromboembolism. Platelet research requires the use of animal models, andmice are widely used in the field. When working , conventional lightin vitrotransmission techniques have been scaled down allowing reduction in animalnumbers. , vascular injury models are widely used and work is ongoing toIn vivodevelop  approaches that use fewer animals. Thromboembolic mortalityex vivomodels, which inflict considerable pain and suffering, have also been usedwidely. A published and characterised refinement of this mortality model allowsreal-time monitoring of radiolabelled platelets under general anaesthesia andreduces both the severity level and the numbers of mice used in a typicalexperiment. This technique is more sensitive than the mortality approach andhas opened up new avenues of research, which would not have been feasibleby using death as an end-point. To drive uptake of real-time monitoring, a moresimplistic approach has been developed involving micro-sampling and cellcounting. Thromboembolic mortality models should therefore be consideredobsolete due to the emergence of 3Rs models with improved scientificoutcomes and that can be implemented relatively easily.
AU  - Taylor,KA
AU  - Emerson,M
DO  - 10.12688/f1000research.14456.1
PY  - 2018///
SN  - 2046-1402
TI  - Refinement of a mouse cardiovascular model: development, application and dissemination
T2  - F1000Research
UR  - http://dx.doi.org/10.12688/f1000research.14456.1
UR  - http://hdl.handle.net/10044/1/59740
VL  - 7
ER  -
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