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@article{Gillespie:2018:10.1111/bph.14368,
author = {Gillespie, S and Holloway, PM and Becker, F and Vital, SA and Taylor, KA and Stokes, KY and Emerson, M and Gavins, FNE},
doi = {10.1111/bph.14368},
journal = {British Journal of Pharmacology},
pages = {3333--3346},
title = {The isothiocyanate sulforaphane modulates platelet function and protects against cerebral thrombotic dysfunction},
url = {http://dx.doi.org/10.1111/bph.14368},
volume = {175},
year = {2018}
}

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TY  - JOUR
AB  - Background and purposePlatelet activation provides a critical link between inflammation and thrombosis. Sulforaphane (SFN), a naturally occurring isothiocyanate, has been shown to display both antiinflammatory and antithrombotic actions in the systemic microvasculature. As inflammation promotes thrombosis and vice versa, this study investigates whether SFN is able to reduce inflammatory potentiation of thrombotic events, suppress platelet activation and thrombus formation in the cerebral microvasculature.Experimental approachThrombosis was induced in the murine brain using the light/dyeinjury model, in conjunction with lipopolysaccharide (LPS) treatment, with and without SFN treatment. In vitro and in vivo platelet assays (aggregation, flow and other functional tests) were also employed, using both human and murine platelets.Key ResultsSFN was found to reduce LPS mediated enhancement of thrombus formation in the cerebral microcirculation. In tail bleed experiments LPS treatment prolonged bleeding time, and SFN treatment was found to protect against this LPSinduced derangement of platelet function. SFN inhibited collagenmediated platelet aggregation in vitro and in vivo and the associated adhesion and impaired calcium signalling. Furthermore, Glycoprotein VI (GPVI) is involved in the protective effects observed with SFN treatment.Conclusions and ImplicationsData presented here provides evidence for the use of SFN in preventing stroke in selected highrisk patient cohorts.
AU  - Gillespie,S
AU  - Holloway,PM
AU  - Becker,F
AU  - Vital,SA
AU  - Taylor,KA
AU  - Stokes,KY
AU  - Emerson,M
AU  - Gavins,FNE
DO  - 10.1111/bph.14368
EP  - 3346
PY  - 2018///
SN  - 1476-5381
SP  - 3333
TI  - The isothiocyanate sulforaphane modulates platelet function and protects against cerebral thrombotic dysfunction
T2  - British Journal of Pharmacology
UR  - http://dx.doi.org/10.1111/bph.14368
UR  - http://hdl.handle.net/10044/1/59839
VL  - 175
ER  -

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