Imperial College London
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DrMikeEmerson

Faculty of Medicine, National Heart & Lung Institute

Reader in Platelet Pharmacology
 
 
 
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Contact

 

+44 (0)7941 828 744m.emerson Website

 
 
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Location

 

537ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cleary:2020:10.1016/j.vascn.2019.106660,
author = {Cleary, SJ and Rauzi, F and Smyth, E and Correia, A and Hobbs, C and Emerson, M and Page, CP and Pitchford, SC},
doi = {10.1016/j.vascn.2019.106660},
journal = {Journal of Pharmacological and Toxicological Methods},
pages = {106660--106660},
title = {Radiolabelling and immunohistochemistry reveal platelet recruitment into lungs and platelet migration into airspaces following LPS inhalation in mice},
url = {http://dx.doi.org/10.1016/j.vascn.2019.106660},
volume = {102},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - INTRODUCTION: Platelets are under investigation for their role in host defence and inflammatory lung diseases and have been demonstrated to be recruited to the lung. However, the mechanisms and consequences of platelet recruitment into lungs are poorly understood. We have utilised a murine model to investigate the mechanisms of platelet involvement in lung inflammation induced by intranasal administration of LPS. OBJECTIVES: Our aim was to characterise lung platelet recruitment following LPS inhalation in mice using immunohistochemistry, and non-invasive and invasive radiolabelled platelet tracking techniques. RESULTS: Intranasal administration of LPS caused an increase in lung platelet staining in lung tissue and elicited the recruitment of radiolabelled platelets into the lung. Prior to these responses in the lung, we observed an earlier decrease in blood platelet counts, temporally associated with platelet recruitment to the liver and spleen. Non-invasive measurements of thoracic radioactivity reflected changes in blood counts rather than extravascular lung platelet recruitment. However, both in situ counting of radiolabelled platelets and immunostaining for platelet surface markers showed LPS-induced increases in extravascular platelets into lung airspaces suggesting that some of the platelets recruited to the lung enter air spaces. CONCLUSIONS: Intranasal administration of LPS activates the innate immune response which includes a fall in peripheral blood platelet counts with subsequent platelet recruitment to the lung, spleen and liver, measured by immunohistochemistry and radiolabelling techniques.
AU  - Cleary,SJ
AU  - Rauzi,F
AU  - Smyth,E
AU  - Correia,A
AU  - Hobbs,C
AU  - Emerson,M
AU  - Page,CP
AU  - Pitchford,SC
DO  - 10.1016/j.vascn.2019.106660
EP  - 106660
PY  - 2020///
SN  - 1056-8719
SP  - 106660
TI  - Radiolabelling and immunohistochemistry reveal platelet recruitment into lungs and platelet migration into airspaces following LPS inhalation in mice
T2  - Journal of Pharmacological and Toxicological Methods
UR  - http://dx.doi.org/10.1016/j.vascn.2019.106660
UR  - https://www.ncbi.nlm.nih.gov/pubmed/31838234
UR  - http://hdl.handle.net/10044/1/76384
VL  - 102
ER  -
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