Imperial College London
Alternate

DrMariaGomez Romero

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Mass Spectrometry and Chromatography Manager
 
 
 
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Contact

 

+44 (0)20 7594 3765m.gomez-romero Website

 
 
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Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Abbassi-Ghadi:2015:10.1007/s13361-015-1278-8,
author = {Abbassi-Ghadi, N and Jones, EA and Gomez-Romero, M and Golf, O and Kumar, S and Huang, J and Kudo, H and Goldin, RD and Hanna, GB and Takats, Z},
doi = {10.1007/s13361-015-1278-8},
journal = {Journal of the American Society for Mass Spectrometry},
pages = {255--264},
title = {A Comparison of DESI-MS and LC-MS for the Lipidomic Profiling of Human Cancer Tissue},
url = {http://dx.doi.org/10.1007/s13361-015-1278-8},
volume = {27},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In this study, we make a direct comparison between desorptionelectrospray ionization-mass spectrometry (DESI-MS) and ultraperformance liquidchromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS) platformsfor the profiling of glycerophospholipid (GPL) species in esophageal cancertissue. In particular, we studied the similarities and differences in the range of GPLsdetected and the congruency of their relative abundances as detected by eachanalytical platform. The main differences between mass spectra of the two modalitieswere found to be associated with the variance in adduct formation of common GPLs,rather than the presence of different GPL species. Phosphatidylcholines as formateadducts in UPLC-ESI-MS accounted for the majority of differences in negative ionmode and alkali metal adducts of phosphatidylcholines in DESI-MS for positive ion mode. Comparison of therelative abundance of GPLs, normalized to a common peak, revealed a correlation coefficient of 0.70 (P < 0.001).The GPL profile detected by DESI-MS is congruent to UPLC-ESI-MS, which reaffirms the role of DESI-MS forlipidomic profiling and a potential premise for quantification.
AU  - Abbassi-Ghadi,N
AU  - Jones,EA
AU  - Gomez-Romero,M
AU  - Golf,O
AU  - Kumar,S
AU  - Huang,J
AU  - Kudo,H
AU  - Goldin,RD
AU  - Hanna,GB
AU  - Takats,Z
DO  - 10.1007/s13361-015-1278-8
EP  - 264
PY  - 2015///
SN  - 1044-0305
SP  - 255
TI  - A Comparison of DESI-MS and LC-MS for the Lipidomic Profiling of Human Cancer Tissue
T2  - Journal of the American Society for Mass Spectrometry
UR  - http://dx.doi.org/10.1007/s13361-015-1278-8
VL  - 27
ER  -
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