Imperial College London
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DrMatthewHind

Faculty of Medicine, National Heart & Lung Institute

Honorary Research Officer
 
 
 
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Contact

 

m.hind

 
 
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Location

 

Royal BromptonRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kim:2020:10.1002/cpmo.85,
author = {Kim, S and Mongey, R and Griffiths, M and Hind, M and Dean, C},
doi = {10.1002/cpmo.85},
journal = {Current protocols in mouse biology},
pages = {e85--e85},
title = {An ex vivo acid injury and repair (AIR) model using precision-cut lung slices to understand lung injury and repair},
url = {http://dx.doi.org/10.1002/cpmo.85},
volume = {10},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Recent advances in cell culture models like airliquid interface culture and ex vivo models such as organoids have advanced studies of lung biology; however, gaps exist between these models and tools that represent the complexity of the threedimensional environment of the lung. Precisioncut lung slices (PCLS) mimic the in vivo environment and bridge the gap between in vitro and in vivo models. We have established the acid injury and repair (AIR) model where a spatially restricted area of tissue is injured using drops of HCl combined with Pluronic gel. Injury and repair are assessed by immunofluorescence using robust markers, including Ki67 for cell proliferation and prosurfactant protein C for alveolar type 2/progenitor cells. Importantly, the AIR model enables the study of injury and repair in mouse lung tissue without the need for an initial in vivo injury, and the results are highly reproducible. Here, we present detailed protocols for the generation of PCLS and the AIR model. We also describe methods to analyze and quantify injury in AIRPCLS by immunostaining with established early repair markers and fluorescence imaging. This novel ex vivo model is a versatile tool for studying lung cell biology in acute lung injury and for semihighthroughput screening of potential therapeutics. © 2020 Wiley Periodicals LLC.
AU  - Kim,S
AU  - Mongey,R
AU  - Griffiths,M
AU  - Hind,M
AU  - Dean,C
DO  - 10.1002/cpmo.85
EP  - 85
PY  - 2020///
SN  - 2161-2617
SP  - 85
TI  - An ex vivo acid injury and repair (AIR) model using precision-cut lung slices to understand lung injury and repair
T2  - Current protocols in mouse biology
UR  - http://dx.doi.org/10.1002/cpmo.85
UR  - http://hdl.handle.net/10044/1/85304
VL  - 10
ER  -
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