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@article{Ledesma:2023:10.1039/D2CB00231K,
author = {Ledesma, Amaro R and Selles, Vidal L and Isalan, M and Heap, J},
doi = {10.1039/D2CB00231K},
journal = {RSC Chemical Biology},
pages = {271--291},
title = {A primer to directed evolution: current methodologies and future directions},
url = {http://dx.doi.org/10.1039/D2CB00231K},
volume = {4},
year = {2023}
}

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TY  - JOUR
AB  - Directed evolution is one of the most powerful tools for protein engineering and functions by harnessing natural evolution, but on a shorter timescale. It enables the rapid selection of variants of biomolecules with properties that make them more suitable for specific applications. Since the first in vitro evolution experiments performed by Sol Spiegelman in 1967, a wide range of techniques have been developed to tackle the main two steps of directed evolution: genetic diversification (library generation), and isolation of the variants of interest. This review covers the main modern methodologies, discussing the advantages and drawbacks of each, and hence the considerations for designing directed evolution experiments. Furthermore, the most recent developments are discussed, showing how advances in the handling of ever larger library sizes are enabling new research questions to be tackled.
AU  - Ledesma,Amaro R
AU  - Selles,Vidal L
AU  - Isalan,M
AU  - Heap,J
DO  - 10.1039/D2CB00231K
EP  - 291
PY  - 2023///
SN  - 2633-0679
SP  - 271
TI  - A primer to directed evolution: current methodologies and future directions
T2  - RSC Chemical Biology
UR  - http://dx.doi.org/10.1039/D2CB00231K
UR  - https://pubs.rsc.org/en/content/articlelanding/2023/CB/D2CB00231K
UR  - http://hdl.handle.net/10044/1/102800
VL  - 4
ER  -

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