Publications

BibTex format

@article{Broedel:2016:10.1038/ncomms13858,
author = {Broedel, AK and Jaramillo, A and Isalan, M},
doi = {10.1038/ncomms13858},
journal = {Nature Communications},
pages = {1--9},
title = {Engineering orthogonal dual transcription factors for multi-input synthetic promoters},
url = {http://dx.doi.org/10.1038/ncomms13858},
volume = {7},
year = {2016}
}

Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Synthetic biology has seen an explosive growth in the capability of engineering artificial gene circuits from transcription factors (TFs), particularly in bacteria. However, most artificial networks still employ the same core set of TFs (for example LacI, TetR and cI). The TFs mostly function via repression and it is difficult to integrate multiple inputs in promoter logic. Here we present to our knowledge the first set of dual activator-repressor switches for orthogonal logic gates, based on bacteriophage λ cI variants and multi-input promoter architectures. Our toolkit contains 12 TFs, flexibly operating as activators, repressors, dual activator–repressors or dual repressor–repressors, on up to 270 synthetic promoters. To engineer non cross-reacting cI variants, we design a new M13 phagemid-based system for the directed evolution of biomolecules. Because cI is used in so many synthetic biology projects, the new set of variants will easily slot into the existing projects of other groups, greatly expanding current engineering capacities.
AU  - Broedel,AK
AU  - Jaramillo,A
AU  - Isalan,M
DO  - 10.1038/ncomms13858
EP  - 9
PY  - 2016///
SN  - 2041-1723
SP  - 1
TI  - Engineering orthogonal dual transcription factors for multi-input synthetic promoters
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/ncomms13858
UR  - https://www.nature.com/articles/ncomms13858
UR  - http://hdl.handle.net/10044/1/41931
VL  - 7
ER  -

Download

Professor Mark Isalan - Deputy Head of Department

Contact

Location

Summary

Citation

BibTex format

RIS format (EndNote, RefMan)