Imperial College London
Alternate

Professor Mark Isalan - Deputy Head of Department

Faculty of Natural Sciences, Department of Life Sciences

Professor of Synthetic Biology
 
 
 
//

Contact

 

+44 (0)20 7594 6482m.isalan

 
 
//

Location

 

509Sir Alexander Fleming BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Broedel:2017:10.1016/j.copbio.2017.11.004,
author = {Broedel, AK and Isalan, M and Jaramillo, A},
doi = {10.1016/j.copbio.2017.11.004},
journal = {Current Opinion in Biotechnology},
pages = {32--38},
title = {Engineering of biomolecules by bacteriophage directed evolution},
url = {http://dx.doi.org/10.1016/j.copbio.2017.11.004},
volume = {51},
year = {2017}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Conventional in vivo directed evolution methods have primarily linked the biomolecule's activity to bacterial cell growth. Recent developments instead rely on the conditional growth of bacteriophages (phages), viruses that infect and replicate within bacteria. Here we review recent phage-based selection systems for in vivo directed evolution. These approaches have been applied to evolve a wide range of proteins including transcription factors, polymerases, proteases, DNA-binding proteins, and protein–protein interactions. Advances in this field expand the possible applications of protein and RNA engineering. This will ultimately result in new biomolecules with tailor-made properties, as well as giving us a better understanding of basic evolutionary processes.
AU  - Broedel,AK
AU  - Isalan,M
AU  - Jaramillo,A
DO  - 10.1016/j.copbio.2017.11.004
EP  - 38
PY  - 2017///
SN  - 0958-1669
SP  - 32
TI  - Engineering of biomolecules by bacteriophage directed evolution
T2  - Current Opinion in Biotechnology
UR  - http://dx.doi.org/10.1016/j.copbio.2017.11.004
UR  - http://hdl.handle.net/10044/1/54017
VL  - 51
ER  -
Download