Imperial College London
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DrMelpomeniKalofonou

Faculty of Engineering, Department of Electrical and Electronic Engineering

Research Fellow in Cancer Technology
 
 
 
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Contact

 

+44 (0)20 7594 1594m.kalofonou Website CV

 
 
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Location

 

B420C - Centre for Bio-Inspired Technology (CBIT)Bessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kottorou:2018:10.18632/oncotarget.25115,
author = {Kottorou, A and Antonacopoulou, A and Dimitrakopoulos, F-I and Diamantopoulou, G and Sirinian, C and Kalofonou, M and Theodorakopoulos, T and Oikonomou, C and Katsakoulis, E and Koutras, A and Makatsoris, T and Demopoulos, N and Stephanou, G and Stavropoulos, M and Thomopoulos, K and Kalofonos, H},
doi = {10.18632/oncotarget.25115},
journal = {Oncotarget},
pages = {21411--21428},
title = {Deregulation of methylation of transcribed-ultra conserved regions in colorectal cancer and their value for detection of adenomas and adenocarcinomas},
url = {http://dx.doi.org/10.18632/oncotarget.25115},
volume = {9},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Expression of Transcribed Ultraconserved Regions (T-UCRs) is often deregulated in cancer. The present study assesses the expression and methylation of three T-UCRs (Uc160, Uc283 and Uc346) in colorectal cancer (CRC) and explores the potential of T-UCR methylation in circulating DNA for the detection of adenomas and adenocarcinomas.Expression levels of Uc160, Uc283 and Uc346 were lower in neoplastic tissues from 64 CRC patients (statistically significant for Uc160, p<0.001), compared to non-malignant tissues, while methylation levels displayed the inverse pattern (p<0.001, p=0.001 and p=0.004 respectively). In colon cancer cell lines, overexpression of Uc160 and Uc346 led to increased proliferation and migration rates. Methylation levels of Uc160 in plasma of 50 CRC, 59 adenoma patients, 40 healthy subjects and 12 patients with colon inflammation or diverticulosis predicted the presence of CRC with 35% sensitivity and 89% specificity (p=0.016), while methylation levels of the combination of all three T-UCRs resulted in 45% sensitivity and 74.3% specificity (p=0.013). In conclusion, studied T-UCRs’ expression and methylation status are deregulated in CRC while Uc160 and Uc346 appear to have a complicated role in CRC progression. Moreover their methylation status appears a promising non-invasive screening test for CRC, provided that the sensitivity of the assay is improved.
AU  - Kottorou,A
AU  - Antonacopoulou,A
AU  - Dimitrakopoulos,F-I
AU  - Diamantopoulou,G
AU  - Sirinian,C
AU  - Kalofonou,M
AU  - Theodorakopoulos,T
AU  - Oikonomou,C
AU  - Katsakoulis,E
AU  - Koutras,A
AU  - Makatsoris,T
AU  - Demopoulos,N
AU  - Stephanou,G
AU  - Stavropoulos,M
AU  - Thomopoulos,K
AU  - Kalofonos,H
DO  - 10.18632/oncotarget.25115
EP  - 21428
PY  - 2018///
SN  - 1949-2553
SP  - 21411
TI  - Deregulation of methylation of transcribed-ultra conserved regions in colorectal cancer and their value for detection of adenomas and adenocarcinomas
T2  - Oncotarget
UR  - http://dx.doi.org/10.18632/oncotarget.25115
UR  - https://doi.org/10.18632/oncotarget.25115
UR  - http://hdl.handle.net/10044/1/59335
VL  - 9
ER  -
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