Imperial College London

Dr Kuimova

Faculty of Natural SciencesDepartment of Chemistry

Reader in Chemical Physics
 
 
 
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Contact

 

+44 (0)20 7594 8558m.kuimova

 
 
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Location

 

207BMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Publication Type
Year
to

104 results found

Davidson N, Tong H-J, Kalberer M, Seville PC, Ward A, Kuimova M, Pope FDet al., 2016, Measurement of the physico-chemical properties of pharmaceutical aerosols as they travel from pressurised metered dose inhalers (PMDI) to a model lung, Journal of Aerosol Medicine and Pulmonary Drug Delivery, Vol: 29, Pages: A25-A25, ISSN: 1941-2711

Journal article

Thanh TV, Meallet-Renault R, Clavier G, Trofimov BA, Kuimova MKet al., 2016, Tuning BODIPY molecular rotors into the red: sensitivity to viscosity vs. temperature, Journal of Materials Chemistry C, Vol: 4, Pages: 2828-2833, ISSN: 2050-7534

Viscosity variations in the microscopic world are of paramount importance for diffusion and reactions. In the last decade a new class of fluorescent probes for measuring viscosity has emerged termed ‘molecular rotors’, which allows quantitative mapping of viscosity in microscopically heterogeneous environments. Here we attempt to tune the absorption and emission of one such ‘molecular rotor’ based on the BODIPY fluorescent core into the red region of the spectrum, to allow better compatibility with the ‘tissue optical window’ and imaging of cells and tissues. We consequently find that our red-emitting BODIPY fluorophores are sensitive to environmental temperature rather than to viscosity, thus suggesting a new prototype for a ‘molecular thermometer’.

Journal article

Vilar Compte R, Shivalingam A, Vysniauskas A, Albrecht T, White AJ, Kuimova MKet al., 2016, Trianguleniums as optical probes for G-quadruplexes: A photophysical, electrochemical and computational study, Chemistry - A European Journal, Vol: 22, Pages: 4129-4139, ISSN: 0947-6539

Nucleic acids can adopt non-duplex topologies such as G-quadruplexes in vitro. Yet it hasbeen challenging to establish their existence and function in vivo due to a lack of suitabletools. Recently, we identified the triangulenium compound DAOTA-M2 as a uniquefluorescence probe for such studies. This probe’s emission lifetime is highly dependent onthe topology of the DNA it interacts with opening up the possibility of carrying out live cellimaging studies. Herein we describe the origin of its fluorescence selectivity for Gquadruplexes.Cyclic voltammetry predicts that the appended morpholino groups can act asintra-molecular photo-induced electron transfer (PET) quenchers. Photophysical studies showthat a delicate balance between this effect and inter-molecular PET with nucleobases is key tothe overall fluorescence enhancement observed upon nucleic acid binding. We utilisedcomputational modelling to demonstrate a conformational dependence of intra-molecularPET. Finally, we performed orthogonal studies with a triangulenium compound where themorpholino groups were removed and demonstrate that this change inverts trianguleniumfluorescence selectivity from G-quadruplex to duplex DNA, thus highlighting the importanceof fine-tuning the molecular structure not only for target affinity but also for fluorescenceresponse.

Journal article

Hosny NA, Fitzgerald C, Vysniauskas A, Athanasiadis A, Berkemeier T, Uygur N, Poeschl U, Shiraiwa M, Kalberer M, Pope FD, Kuimova MKet al., 2015, Direct imaging of changes in aerosol particle viscosity upon hydration and chemical aging, Chemical Science, Vol: 7, Pages: 1357-1367, ISSN: 2041-6539

Organic aerosol particles (OA) play major roles in atmospheric chemistry, climate, and public health. Aerosol particle viscosity is highly important since it can determine the ability of chemical species such as oxidants, organics or water to diffuse into the particle bulk. Recent measurements indicate that OA may be present in highly viscous states, however, diffusion rates of small molecules such as water are not limited by these high viscosities. Direct observational evidence of kinetic barriers caused by high viscosity and low diffusivity in aerosol particles were not available until recently; and techniques that are able to dynamically quantify and track viscosity changes during atmospherically relevant processes are still unavailable for atmospheric aerosols. Here we report quantitative, real-time, online observations of microscopic viscosity changes in aerosol particles of atmospherically relevant composition, using fluorescence lifetime imaging (FLIM) of viscosity. We show that microviscosity in ozonated oleic acid droplets and secondary organic aerosol (SOA) particles formed by ozonolysis of myrcene increases substantially with decreasing humidity and atmospheric oxidative aging processes. Furthermore, we found unexpected heterogeneities of microviscosity inside individual aerosol particles. The results of this study enhance our understanding of organic aerosol processes on microscopic scales and may have important implications for the modeling of atmospheric aerosol growth, composition and interactions with trace gases and clouds.

Journal article

Shivalingam A, Izquierdo MA, Le Marois A, Vyšniauskas A, Suhling K, Kuimova MK, Vilar Ret al., 2015, The interactions between a small molecule and G-quadruplexes are visualised by fluorescence lifetime imaging microscopy, Nature Communications, Vol: 6, ISSN: 2041-1723

Guanine-rich oligonucleotides can fold into quadruple-stranded helical structures known as G-quadruplexes. Mounting experimental evidence has gathered suggesting that these non-canonical nucleic acid structures form in vivo and play essential biological roles. However, to date, there are no small-molecule optical probes to image G-quadruplexes in live cells. Herein, we report the design and development of a small fluorescent molecule, which can be used as an optical probe for G-quadruplexes. We demonstrate that the fluorescence lifetime of this new probe changes considerably upon interaction with different nucleic acid topologies. Specifically, longer fluorescence lifetimes are observed in vitro for G-quadruplexes than for double- and single-stranded nucleic acids. Cellular studies confirm that this molecule is cell permeable, has low cytotoxicity and localizes primarily in the cell nucleus. Furthermore, using fluorescence lifetime imaging microscopy, live-cell imaging suggests that the probe can be used to study the interaction of small molecules with G-quadruplexes in vivo.

Journal article

Thompson AJ, Herling TW, Kubankova M, Vysniauskas A, Knowles TP, Kuimova MKet al., 2015, Molecular Rotors Provide Insights into Microscopic Structural Changes During Protein Aggregation., Journal of Physical Chemistry B, Vol: 119, Pages: 10170-10179, ISSN: 1520-6106

Changes in microscopic viscosity represent an important characteristic of structural transitions in soft matter systems. Here we demonstrate the use of molecular rotors to explore the changes in microrheology accompanying the transition of proteins from their soluble states into a gel phase composed of amyloid fibrils. The formation of beta-sheet rich protein aggregates, including amyloid fibrils, is a hallmark of a number of neurodegenerative disorders, and as such, the mechanistic details of this process are actively sought after. In our experiments, molecular rotors report an increase in rigidity of approximately three orders of magnitude during the aggregation reaction. Moreover, phasor analysis of the fluorescence decay signal from the molecular rotors suggests the presence of multiple distinct mechanistic stages during the aggregation process. Our results show that molecular rotors can reveal key microrheological features of protein systems not observable through classical fluorescent probes operating in light switch mode.

Journal article

Vysniauskas A, Qurashi M, Gallop N, Balaz M, Anderson HL, Kuimova MKet al., 2015, Unravelling the effect of temperature on viscosity-sensitive fluorescent molecular rotors, CHEMICAL SCIENCE, Vol: 6, Pages: 5773-5778, ISSN: 2041-6520

Viscosity and temperature variations in the microscopic world are of paramount importance for diffusion and reactions. Consequently, a plethora of fluorescent probes have evolved over the years to enable fluorescent imaging of both parameters in biological cells. However, the simultaneous effect of both temperature and viscosity on the photophysical behavior of fluorophores is rarely considered, yet unavoidable variations in temperature can lead to significant errors in the readout of viscosity and vice versa. Here we examine the effect of temperature on the photophysical behavior of three classes of viscosity-sensitive fluorophores termed ‘molecular rotors’. For each of the fluorophores we decouple the effect of temperature from the effect of viscosity. In the case of the conjugated porphyrin dimer, we demonstrate that, uniquely, simultaneous dual-mode lifetime and intensity measurements of this fluorophore can be used for measuring both viscosity and temperature concurrently.

Journal article

Kubankova M, Kuimova M, 2015, Molecular rotors as novel probes of protein aggregation, EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, Vol: 44, Pages: S80-S80, ISSN: 0175-7571

Journal article

Dent M, Lopez-Duarte I, Bull J, Brooks NJ, Kuimova Met al., 2015, Imaging patterns in lipid membranes through the use of molecular rotors, EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, Vol: 44, Pages: S107-S107, ISSN: 0175-7571

Journal article

Mika JT, Thompson AJ, Hofkens J, Kuimova MKet al., 2015, Measurement of the Viscosity of E. coli Membranes using Molecular Rotors and Flim, 59th Annual Meeting of the Biophysical-Society, Publisher: CELL PRESS, Pages: 542A-542A, ISSN: 0006-3495

Conference paper

Lopez-Duarte I, Chairatana P, Wu Y, Perez-Moreno J, Bennett PM, Reeve JE, Boczarow I, Kaluza W, Hosny NA, Stranks SD, Nicholas RJ, Clays K, Kuimova MK, Anderson HLet al., 2015, Thiophene-based dyes for probing membranes, ORGANIC & BIOMOLECULAR CHEMISTRY, Vol: 13, Pages: 3792-3802, ISSN: 1477-0520

Journal article

Vysniauskas A, Balaz M, Anderson HL, Kuimova MKet al., 2015, Dual mode quantitative imaging of microscopic viscosity using a conjugated porphyrin dimer, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 17, Pages: 7548-7554, ISSN: 1463-9076

Journal article

Dent MR, Lopez-Duarte I, Dickson CJ, Geoghegan ND, Cooper JM, Gould IR, Krams R, Bull JA, Brooks NJ, Kuimova MKet al., 2015, Imaging phase separation in model lipid membranes through the use of BODIPY based molecular rotors, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 17, Pages: 18393-18402, ISSN: 1463-9076

Journal article

Izquierdo MA, Vyšniauskas A, Lermontova SA, Grigoryev IS, Shilyagina NY, Balalaeva IV, Klapshina LG, Kuimova MKet al., 2015, Dual use of porphyrazines as sensitizers and viscosity markers in photodynamic therapy, J. Mater. Chem. B, Vol: 3, Pages: 1089-1096, ISSN: 2050-750X

Journal article

Richardson RD, Baud MGJ, Weston CE, Rzepa HS, Kuimova MK, Fuchter MJet al., 2015, Dual wavelength asymmetric photochemical synthesis with circularly polarized light, Chemical Science, Vol: 6, Pages: 3853-3862, ISSN: 2041-6539

Journal article

Tang T-YD, Hak CRC, Thompson AJ, Kuimova MK, Williams DS, Perriman AW, Mann Set al., 2014, Fatty acid membrane assembly on coacervate microdroplets as a step towards a hybrid protocell model, NATURE CHEMISTRY, Vol: 6, Pages: 527-533, ISSN: 1755-4330

Journal article

Lopez-Duarte I, Thanh TV, Izquierdo MA, Bull JA, Kuimova MKet al., 2014, A molecular rotor for measuring viscosity in plasma membranes of live cells, CHEMICAL COMMUNICATIONS, Vol: 50, Pages: 5282-5284, ISSN: 1359-7345

Journal article

Thompson AJ, Tang T-YD, Herling TW, Hak CRC, Mann S, Knowles TPJ, Kuimova MKet al., 2014, Quantitative sensing of microviscosity in protocells and amyloid materials using fluorescence lifetime imaging of molecular rotors, Conference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, Publisher: SPIE- Society of Photo-optical Instrumentation Engineers, ISSN: 0277-786X

Molecular rotors are fluorophores that have a fluorescence quantum yield that depends upon intermolecular rotation. The fluorescence quantum yield, intensity and lifetime of molecular rotors all vary as functions of viscosity, as high viscosities inhibit intermolecular rotation and cause an increase in the non-radiative decay rate. As such, molecular rotors can be used to probe viscosity on microscopic scales. Here, we apply fluorescence lifetime imaging microscopy (FLIM) to measure the fluorescence lifetimes of three different molecular rotors, in order to determine the microscopic viscosity in two model systems with significant biological interest. First, the constituents of a novel protocell – a model of a prebiotic cell – were studied using the molecular rotors BODIPY C10 and kiton red. Second, amyloid formation was investigated using the molecular rotor Cy3. © (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.

Conference paper

Tong H-J, Fitzgerald C, Gallimore PJ, Kalberer M, Kuimova MK, Seville PC, Ward AD, Pope FDet al., 2014, Rapid interrogation of the physical and chemical characteristics of salbutamol sulphate aerosol from a pressurised metered-dose inhaler (pMDI), CHEMICAL COMMUNICATIONS, Vol: 50, Pages: 15499-15502, ISSN: 1359-7345

Journal article

Karim NHA, Mendoza O, Shivalingam A, Thompson AJ, Ghosh S, Kuimova MK, Vilar Ret al., 2014, Salphen metal complexes as tunable G-quadruplex binders and optical probes, RSC ADVANCES, Vol: 4, Pages: 3355-3363, ISSN: 2046-2069

Journal article

Loison P, Hosny NA, Gervais P, Champion D, Kuimova MK, Perrier-Cornet J-Met al., 2013, Direct investigation of viscosity of an atypical inner membrane of Bacillus spores: A molecular rotor/FLIM study, BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, Vol: 1828, Pages: 2436-2443, ISSN: 0005-2736

Journal article

Kuimova MK, Phillips D, 2013, Photomedicine, Applied Photochemistry, Pages: 331-347, ISBN: 9789048138296

© 2013 Springer Science+Business Media Dordrecht. All rights reserved. This chapter discusses the various modalities of photomedicine, an interdisciplinary branch of medicine that involves the study and application of light with respect to health and disease. The following main concepts are covered: Photodynamic Therapy (PDT) for the treatment of cancer, PDT for bacterial infections, vascular PDT, photochemical internalisation, photochemical tissue bonding and the use of lasers in medicine.

Book chapter

Hosny NA, Mohamedi G, Rademeyer P, Owen J, Wu Y, Tang M-X, Eckersley RJ, Stride E, Kuimova MKet al., 2013, Mapping microbubble viscosity using fluorescence lifetime imaging of molecular rotors, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: 9225-9230, ISSN: 0027-8424

Journal article

Mohamedi G, Hosny NA, Rademeyer P, Park Y, Owen J, Pham T, Wong JY, Kuimova M, Stride Eet al., 2013, Investigating the effect of fabrication method on the stability and acoustic response of microbubble agents., J Acoust Soc Am, Vol: 133

Microbubbles stabilized by a surfactant or polymer coating are already in clinical use as ultrasound imaging contrast agents. They have also been widely investigated as vehicles for drug delivery and gene therapy that can be tracked and triggered using ultrasound. Extensive studies have been made of the effects of the coating material and gas core on microbubble characteristics, but the influence of the fabrication method has received less attention. The aim of this study was to compare the behavior of microbubbles prepared using different techniques. Phospholipid-coated microbubbles were produced using sonication, electrospraying, or in a specially designed microfluidic device. The microbubbles were observed using optical, electron, and fluorescence lifetime imaging microscopy (FLIM) to interrogate their surface microstructure and stability over time. Their acoustic response was then determined in a flow chamber by detecting the pressure scattered from individual microbubbles as they passed through the focal region of a transducer (center frequencies 1, 2.25, and 3.5 MHz; peak negative pressures 50-300 kPa). The method of bubble generation was found to significantly affect the bubble surface characteristics, stability, and acoustic response. The results demonstrate that the processing method affects not only the bubble size distribution but other characteristics important for biomedical applications.

Journal article

Mohamedi G, Hosny NA, Rademeyer P, Park Y, Owen J, Pham T, Wong JY, Kuimova M, Stride Eet al., 2013, Influence of fabrication method on functional properties of microbubble agents for theranostic applications, Symp on Physicochemical Characterization of Organic Matter: Past, Present, Future, and Role of Environment / 245th Natl Spring Meeting of the Amer-Chem-Soc (ACS), Publisher: AMER CHEMICAL SOC, ISSN: 0065-7727

Conference paper

Hosny NA, Fitzgerald C, Tong C, Kalberer M, Kuimova MK, Pope FDet al., 2013, Fluorescent lifetime imaging of atmospheric aerosols: a direct probe of aerosol viscosity, FARADAY DISCUSSIONS, Vol: 165, Pages: 343-356, ISSN: 1359-6640

Journal article

Wu Y, Stefl M, Olzynska A, Hof M, Yahioglu G, Yip P, Casey DR, Ces O, Humpolickova J, Kuimova MKet al., 2013, Molecular rheometry: direct determination of viscosity in L-o and L-d lipid phases via fluorescence lifetime imaging, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 15, Pages: 14986-14993, ISSN: 1463-9076

Journal article

da Silva EFF, Pedersen BW, Breitenbach T, Toftegaard R, Kuimova MK, Arnaut LG, Ogilby PRet al., 2012, Irradiation- and Sensitizer-Dependent Changes in the Lifetime of Intracellular Singlet Oxygen Produced in a Photosensitized Process (vol 116, pg 445, 2012), JOURNAL OF PHYSICAL CHEMISTRY B, Vol: 116, Pages: 14734-14734, ISSN: 1520-6106

Journal article

Suntharalingam K, Leczkowska A, Furrer MA, Wu Y, Kuimova MK, Therrien B, White AJP, Vilar Ret al., 2012, A Cyclometallated Platinum Complex as a Selective Optical Switch for Quadruplex DNA, CHEMISTRY-A EUROPEAN JOURNAL, Vol: 18, Pages: 16277-16282, ISSN: 0947-6539

Journal article

Mahmood T, Wu Y, Loriot D, Kuimova M, Ladame Set al., 2012, Closing the ring to bring up the light: synthesis of a hexacyclic acridinium cyanine dye., Chemistry, Vol: 18, Pages: 12349-12356

The synthesis of a geometrically constrained and near-planar hexacyclic acridinium cyanine dye 9 is reported. When compared to its unlocked and non-fluorescent monomethine cyanine dye analogue 3, this photostable dye emits in the green area of the spectrum with a remarkable quantum yield close to unity in organic solvents and above 0.5 in water. A detailed steady-state and time-resolved spectroscopic study revealed that dye 9 forms emissive aggregates in water, which are responsible for a red-shifted and broadened emission band and longer emission lifetime, τ≈33 compared to 6.5-7.0 ns for the monomeric dye. Dye 9 also binds strongly to DNA (both duplex and quadruplex) in its monomeric form and is very efficiently taken up by cells, in which it accumulates primarily into the nucleus.

Journal article

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