Imperial College London

Dr Kuimova

Faculty of Natural SciencesDepartment of Chemistry

Reader in Chemical Physics
 
 
 
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Contact

 

+44 (0)20 7594 8558m.kuimova

 
 
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Location

 

207BMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Loison:2016:10.1016/j.bbamem.2016.06.003,
author = {Loison, P and Gervais, P and Perrier-Cornet, JM and Kuimova, MK},
doi = {10.1016/j.bbamem.2016.06.003},
journal = {Biochimica et Biophysica Acta},
pages = {2060--2069},
title = {Effect of ethanol perturbation on viscosity and permeability of an inner membrane in Bacillus subtilis spores.},
url = {http://dx.doi.org/10.1016/j.bbamem.2016.06.003},
volume = {1858},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - In this work, we investigated how a combination of ethanol and high temperature (70°C), affect the properties of the inner membrane of Bacillus subtilis spores. We observed membrane permeabilization for ethanol concentrations ≥50%, as indicated by the staining of the spores' DNA by the cell impermeable dye Propidium Iodide. The loss of membrane integrity was also confirmed by a decrease in the peak corresponding to dipicolinic acid using infrared spectroscopy. Finally, the spore refractivity (as measured by phase contrast microscopy) was decreased after the ethanol-heat treatment, suggesting a partial rehydration of the protoplast. Previously we have used fluorescent lifetime imaging microscopy (FLIM) combined with the fluorescent molecular rotor Bodipy-C12 to study the microscopic viscosity in the inner membrane of B. subtilis spores, and showed that at normal conditions it is characterized by a very high viscosity. Here we demonstrate that the ethanol/high temperature treatment led to a decrease of the viscosity of the inner membrane, from 1000cP to 860cP for wild type spores at 50% of ethanol. Altogether, our present work confirms the deleterious effect of ethanol on the structure of B. subtilis spores, as well as demonstrates the ability of FLIM - Bodipy-C12 to measure changes in the microviscosity of the spores upon perturbation.
AU - Loison,P
AU - Gervais,P
AU - Perrier-Cornet,JM
AU - Kuimova,MK
DO - 10.1016/j.bbamem.2016.06.003
EP - 2069
PY - 2016///
SN - 0006-3002
SP - 2060
TI - Effect of ethanol perturbation on viscosity and permeability of an inner membrane in Bacillus subtilis spores.
T2 - Biochimica et Biophysica Acta
UR - http://dx.doi.org/10.1016/j.bbamem.2016.06.003
UR - http://hdl.handle.net/10044/1/34362
VL - 1858
ER -