Imperial College London

Dr Kuimova

Faculty of Natural SciencesDepartment of Chemistry

Reader in Chemical Physics
 
 
 
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Contact

 

+44 (0)20 7594 8558m.kuimova

 
 
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Location

 

207BMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Vysniauskas:2016:10.1002/chem.201601925,
author = {Vysniauskas, A and Qurashi, M and Kuimova, MK},
doi = {10.1002/chem.201601925},
journal = {Chemistry-A European Journal},
pages = {13210--13217},
title = {A Molecular Rotor that Measures Dynamic Changes of Lipid Bilayer Viscosity Caused by Oxidative Stress},
url = {http://dx.doi.org/10.1002/chem.201601925},
volume = {22},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Oxidation of cellular structures is typically an undesirable process that can be a hallmark of certain diseases. On the other hand, photooxidation is a necessary step of photodynamic therapy (PDT), a cancer treatment causing cell death upon light irradiation. Here, the effect of photooxidation on the microscopic viscosity of model lipid bilayers constructed of 1,2-dioleoyl-sn-glycero-3-phosphocholine has been studied. A molecular rotor has been employed that displays a viscosity-dependent fluorescence lifetime as a quantitative probe of the bilayer's viscosity. Thus, spatially-resolved viscosity maps of lipid photooxidation in giant unilamellar vesicles (GUVs) were obtained, testing the effect of the positioning of the oxidant relative to the rotor in the bilayer. It was found that PDT has a strong impact on viscoelastic properties of lipid bilayers, which ‘travels’ through the bilayer to areas that have not been irradiated directly. A dramatic difference in viscoelastic properties of oxidized GUVs by TypeI (electron transfer) and TypeII (singlet oxygen-based) photosensitisers was also detected.
AU - Vysniauskas,A
AU - Qurashi,M
AU - Kuimova,MK
DO - 10.1002/chem.201601925
EP - 13217
PY - 2016///
SN - 1521-3765
SP - 13210
TI - A Molecular Rotor that Measures Dynamic Changes of Lipid Bilayer Viscosity Caused by Oxidative Stress
T2 - Chemistry-A European Journal
UR - http://dx.doi.org/10.1002/chem.201601925
UR - http://hdl.handle.net/10044/1/34052
VL - 22
ER -