Imperial College London

Dr Kuimova

Faculty of Natural SciencesDepartment of Chemistry

Reader in Chemical Physics
 
 
 
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Contact

 

+44 (0)20 7594 8558m.kuimova

 
 
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Location

 

207BMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Chambers:2018:10.1021/acsnano.8b00177,
author = {Chambers, JE and Kubánková, M and Huber, RG and López-Duarte, I and Avezov, E and Bond, PJ and Marciniak, SJ and Kuimova, MK},
doi = {10.1021/acsnano.8b00177},
journal = {ACS Nano},
pages = {4398--4407},
title = {An Optical Technique for Mapping Microviscosity Dynamics in Cellular Organelles.},
url = {http://dx.doi.org/10.1021/acsnano.8b00177},
volume = {12},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Microscopic viscosity (microviscosity) is a key determinant of diffusion in the cell and defines the rate of biological processes occurring at the nanoscale, including enzyme-driven metabolism and protein folding. Here we establish a rotor-based organelle viscosity imaging (ROVI) methodology that enables real-time quantitative mapping of cell microviscosity. This approach uses environment-sensitive dyes termed molecular rotors, covalently linked to genetically encoded probes to provide compartment-specific microviscosity measurements via fluorescence lifetime imaging. ROVI visualized spatial and temporal dynamics of microviscosity with suborganellar resolution, reporting on a microviscosity difference of nearly an order of magnitude between subcellular compartments. In the mitochondrial matrix, ROVI revealed several striking findings: a broad heterogeneity of microviscosity among individual mitochondria, unparalleled resilience to osmotic stress, and real-time changes in microviscosity during mitochondrial depolarization. These findings demonstrate the use of ROVI to explore the biophysical mechanisms underlying cell biological processes.
AU - Chambers,JE
AU - Kubánková,M
AU - Huber,RG
AU - López-Duarte,I
AU - Avezov,E
AU - Bond,PJ
AU - Marciniak,SJ
AU - Kuimova,MK
DO - 10.1021/acsnano.8b00177
EP - 4407
PY - 2018///
SN - 1936-0851
SP - 4398
TI - An Optical Technique for Mapping Microviscosity Dynamics in Cellular Organelles.
T2 - ACS Nano
UR - http://dx.doi.org/10.1021/acsnano.8b00177
UR - https://www.ncbi.nlm.nih.gov/pubmed/29648785
UR - http://hdl.handle.net/10044/1/59933
VL - 12
ER -