Imperial College London

Dr Kuimova

Faculty of Natural SciencesDepartment of Chemistry

Reader in Chemical Physics



+44 (0)20 7594 8558m.kuimova




207BMolecular Sciences Research HubWhite City Campus






BibTex format

author = {Chambers, JE and Kubánková, M and Huber, RG and López-Duarte, I and Avezov, E and Bond, PJ and Marciniak, SJ and Kuimova, MK},
doi = {10.1021/acsnano.8b00177},
journal = {ACS Nano},
pages = {4398--4407},
title = {An Optical Technique for Mapping Microviscosity Dynamics in Cellular Organelles.},
url = {},
volume = {12},
year = {2018}

RIS format (EndNote, RefMan)

AB - Microscopic viscosity (microviscosity) is a key determinant of diffusion in the cell and defines the rate of biological processes occurring at the nanoscale, including enzyme-driven metabolism and protein folding. Here we establish a rotor-based organelle viscosity imaging (ROVI) methodology that enables real-time quantitative mapping of cell microviscosity. This approach uses environment-sensitive dyes termed molecular rotors, covalently linked to genetically encoded probes to provide compartment-specific microviscosity measurements via fluorescence lifetime imaging. ROVI visualized spatial and temporal dynamics of microviscosity with suborganellar resolution, reporting on a microviscosity difference of nearly an order of magnitude between subcellular compartments. In the mitochondrial matrix, ROVI revealed several striking findings: a broad heterogeneity of microviscosity among individual mitochondria, unparalleled resilience to osmotic stress, and real-time changes in microviscosity during mitochondrial depolarization. These findings demonstrate the use of ROVI to explore the biophysical mechanisms underlying cell biological processes.
AU - Chambers,JE
AU - Kubánková,M
AU - Huber,RG
AU - López-Duarte,I
AU - Avezov,E
AU - Bond,PJ
AU - Marciniak,SJ
AU - Kuimova,MK
DO - 10.1021/acsnano.8b00177
EP - 4407
PY - 2018///
SN - 1936-0851
SP - 4398
TI - An Optical Technique for Mapping Microviscosity Dynamics in Cellular Organelles.
T2 - ACS Nano
UR -
UR -
UR -
VL - 12
ER -