Publications
127 results found
Kubankova M, Kuimova M, 2015, Molecular rotors as novel probes of protein aggregation, EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, Vol: 44, Pages: S80-S80, ISSN: 0175-7571
Dent M, Lopez-Duarte I, Bull J, et al., 2015, Imaging patterns in lipid membranes through the use of molecular rotors, EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, Vol: 44, Pages: S107-S107, ISSN: 0175-7571
Mika JT, Thompson AJ, Hofkens J, et al., 2015, Measurement of the Viscosity of E. coli Membranes using Molecular Rotors and Flim, 59th Annual Meeting of the Biophysical-Society, Publisher: CELL PRESS, Pages: 542A-542A, ISSN: 0006-3495
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- Citations: 1
Dent MR, Lopez-Duarte I, Dickson CJ, et al., 2015, Imaging phase separation in model lipid membranes through the use of BODIPY based molecular rotors, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 17, Pages: 18393-18402, ISSN: 1463-9076
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- Citations: 73
Izquierdo MA, Vyšniauskas A, Lermontova SA, et al., 2015, Dual use of porphyrazines as sensitizers and viscosity markers in photodynamic therapy, J. Mater. Chem. B, Vol: 3, Pages: 1089-1096, ISSN: 2050-750X
Lopez-Duarte I, Chairatana P, Wu Y, et al., 2015, Thiophene-based dyes for probing membranes, ORGANIC & BIOMOLECULAR CHEMISTRY, Vol: 13, Pages: 3792-3802, ISSN: 1477-0520
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- Citations: 37
Vysniauskas A, Balaz M, Anderson HL, et al., 2015, Dual mode quantitative imaging of microscopic viscosity using a conjugated porphyrin dimer, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 17, Pages: 7548-7554, ISSN: 1463-9076
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- Citations: 38
Richardson RD, Baud MGJ, Weston CE, et al., 2015, Dual wavelength asymmetric photochemical synthesis with circularly polarized light, Chemical Science, Vol: 6, Pages: 3853-3862, ISSN: 2041-6539
Lopez-Duarte I, Thanh TV, Izquierdo MA, et al., 2014, A molecular rotor for measuring viscosity in plasma membranes of live cells, CHEMICAL COMMUNICATIONS, Vol: 50, Pages: 5282-5284, ISSN: 1359-7345
Tang T-YD, Hak CRC, Thompson AJ, et al., 2014, Fatty acid membrane assembly on coacervate microdroplets as a step towards a hybrid protocell model, Nature Chemistry, Vol: 6, Pages: 527-533, ISSN: 1755-4330
Mechanisms of prebiotic compartmentalization are central to providing insights into how protocellular systems emerged on the early Earth. Protocell models are based predominantly on the membrane self-assembly of fatty-acid vesicles, although membrane-free scenarios that involve liquid–liquid microphase separation (coacervation) have also been considered. Here we integrate these alternative models of prebiotic compartmentalization and develop a hybrid protocell model based on the spontaneous self-assembly of a continuous fatty-acid membrane at the surface of preformed coacervate microdroplets prepared from cationic peptides/polyelectrolytes and adenosine triphosphate or oligo/polyribonucleotides. We show that the coacervate-supported membrane is multilamellar, and mediates the selective uptake or exclusion of small and large molecules. The coacervate interior can be disassembled without loss of membrane integrity, and fusion and growth of the hybrid protocells can be induced under conditions of high ionic strength. Our results highlight how notions of membrane-mediated compartmentalization, chemical enrichment and internalized structuration can be integrated in protocell models via simple chemical and physical processes.
Thompson AJ, Tang T-YD, Herling TW, et al., 2014, Quantitative sensing of microviscosity in protocells and amyloid materials using fluorescence lifetime imaging of molecular rotors, Conference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, Publisher: SPIE- Society of Photo-optical Instrumentation Engineers, ISSN: 0277-786X
Molecular rotors are fluorophores that have a fluorescence quantum yield that depends upon intermolecular rotation. The fluorescence quantum yield, intensity and lifetime of molecular rotors all vary as functions of viscosity, as high viscosities inhibit intermolecular rotation and cause an increase in the non-radiative decay rate. As such, molecular rotors can be used to probe viscosity on microscopic scales. Here, we apply fluorescence lifetime imaging microscopy (FLIM) to measure the fluorescence lifetimes of three different molecular rotors, in order to determine the microscopic viscosity in two model systems with significant biological interest. First, the constituents of a novel protocell – a model of a prebiotic cell – were studied using the molecular rotors BODIPY C10 and kiton red. Second, amyloid formation was investigated using the molecular rotor Cy3. © (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Tong H-J, Fitzgerald C, Gallimore PJ, et al., 2014, Rapid interrogation of the physical and chemical characteristics of salbutamol sulphate aerosol from a pressurised metered-dose inhaler (pMDI), CHEMICAL COMMUNICATIONS, Vol: 50, Pages: 15499-15502, ISSN: 1359-7345
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- Citations: 16
Karim NHA, Mendoza O, Shivalingam A, et al., 2014, Salphen metal complexes as tunable G-quadruplex binders and optical probes, RSC ADVANCES, Vol: 4, Pages: 3355-3363, ISSN: 2046-2069
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- Citations: 51
Loison P, Hosny NA, Gervais P, et al., 2013, Direct investigation of viscosity of an atypical inner membrane of <i>Bacillus</i> spores: A molecular rotor/FLIM study, BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, Vol: 1828, Pages: 2436-2443, ISSN: 0005-2736
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- Citations: 39
Kuimova MK, Phillips D, 2013, Photomedicine, Applied Photochemistry, Pages: 331-347, ISBN: 9789048138296
This chapter discusses the various modalities of photomedicine, an interdisciplinary branch of medicine that involves the study and application of light with respect to health and disease. The following main concepts are covered: Photodynamic Therapy (PDT) for the treatment of cancer, PDT for bacterial infections, vascular PDT, photochemical internalisation, photochemical tissue bonding and the use of lasers in medicine.
Hosny NA, Mohamedi G, Rademeyer P, et al., 2013, Mapping microbubble viscosity using fluorescence lifetime imaging of molecular rotors, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: 9225-9230, ISSN: 0027-8424
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- Citations: 112
Mohamedi G, Hosny NA, Rademeyer P, et al., 2013, Investigating the effect of fabrication method on the stability and acoustic response of microbubble agents., J Acoust Soc Am, Vol: 133
Microbubbles stabilized by a surfactant or polymer coating are already in clinical use as ultrasound imaging contrast agents. They have also been widely investigated as vehicles for drug delivery and gene therapy that can be tracked and triggered using ultrasound. Extensive studies have been made of the effects of the coating material and gas core on microbubble characteristics, but the influence of the fabrication method has received less attention. The aim of this study was to compare the behavior of microbubbles prepared using different techniques. Phospholipid-coated microbubbles were produced using sonication, electrospraying, or in a specially designed microfluidic device. The microbubbles were observed using optical, electron, and fluorescence lifetime imaging microscopy (FLIM) to interrogate their surface microstructure and stability over time. Their acoustic response was then determined in a flow chamber by detecting the pressure scattered from individual microbubbles as they passed through the focal region of a transducer (center frequencies 1, 2.25, and 3.5 MHz; peak negative pressures 50-300 kPa). The method of bubble generation was found to significantly affect the bubble surface characteristics, stability, and acoustic response. The results demonstrate that the processing method affects not only the bubble size distribution but other characteristics important for biomedical applications.
Mohamedi G, Hosny NA, Rademeyer P, et al., 2013, Influence of fabrication method on functional properties of microbubble agents for theranostic applications, Symp on Physicochemical Characterization of Organic Matter: Past, Present, Future, and Role of Environment / 245th Natl Spring Meeting of the Amer-Chem-Soc (ACS), Publisher: AMER CHEMICAL SOC, ISSN: 0065-7727
Hosny NA, Fitzgerald C, Tong C, et al., 2013, Fluorescent lifetime imaging of atmospheric aerosols: a direct probe of aerosol viscosity, FARADAY DISCUSSIONS, Vol: 165, Pages: 343-356, ISSN: 1359-6640
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- Citations: 59
Wu Y, Stefl M, Olzynska A, et al., 2013, Molecular rheometry: direct determination of viscosity in L<sub>o</sub> and L<sub>d</sub> lipid phases <i>via</i> fluorescence lifetime imaging, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 15, Pages: 14986-14993, ISSN: 1463-9076
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- Citations: 137
da Silva EFF, Pedersen BW, Breitenbach T, et al., 2012, Irradiation- and Sensitizer-Dependent Changes in the Lifetime of Intracellular Singlet Oxygen Produced in a Photosensitized Process (vol 116, pg 445, 2012), JOURNAL OF PHYSICAL CHEMISTRY B, Vol: 116, Pages: 14734-14734, ISSN: 1520-6106
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- Citations: 1
Suntharalingam K, Leczkowska A, Furrer MA, et al., 2012, A Cyclometallated Platinum Complex as a Selective Optical Switch for Quadruplex DNA, CHEMISTRY-A EUROPEAN JOURNAL, Vol: 18, Pages: 16277-16282, ISSN: 0947-6539
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- Citations: 50
Mahmood T, Wu Y, Loriot D, et al., 2012, Closing the ring to bring up the light: synthesis of a hexacyclic acridinium cyanine dye., Chemistry, Vol: 18, Pages: 12349-12356
The synthesis of a geometrically constrained and near-planar hexacyclic acridinium cyanine dye 9 is reported. When compared to its unlocked and non-fluorescent monomethine cyanine dye analogue 3, this photostable dye emits in the green area of the spectrum with a remarkable quantum yield close to unity in organic solvents and above 0.5 in water. A detailed steady-state and time-resolved spectroscopic study revealed that dye 9 forms emissive aggregates in water, which are responsible for a red-shifted and broadened emission band and longer emission lifetime, τ≈33 compared to 6.5-7.0 ns for the monomeric dye. Dye 9 also binds strongly to DNA (both duplex and quadruplex) in its monomeric form and is very efficiently taken up by cells, in which it accumulates primarily into the nucleus.
Suhling K, Levitt JA, Chung P-H, et al., 2012, Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells, JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, ISSN: 1940-087X
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- Citations: 9
da Silva EFF, Pedersen BW, Breitenbach T, et al., 2012, Irradiation- and Sensitizer-Dependent Changes in the Lifetime of Intracellular Singlet Oxygen Produced in a Photosensitized Process, JOURNAL OF PHYSICAL CHEMISTRY B, Vol: 116, Pages: 445-461, ISSN: 1520-6106
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- Citations: 88
Kuimova MK, 2012, Molecular Rotors Image Intracellular Viscosity, CHIMIA, Vol: 66, Pages: 159-165, ISSN: 0009-4293
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- Citations: 27
Kuimova MK, 2012, Mapping viscosity in cells using molecular rotors, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 14, Pages: 12671-12686, ISSN: 1463-9076
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- Citations: 350
Kuimova MK, 2011, Measuring intracellular viscosity: from molecular rotors to Photodynamic Therapy of cancer, 8th EBSA European Biophysics Congress, Publisher: SPRINGER, Pages: 150-150, ISSN: 0175-7571
Levitt JA, Chung P-H, Kuimova MK, et al., 2011, Fluorescence Anisotropy of Molecular Rotors, CHEMPHYSCHEM, Vol: 12, Pages: 662-672, ISSN: 1439-4235
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- Citations: 96
Chung P-H, Levitt JA, Kuimova MK, et al., 2011, Mapping intracellular viscosity by advanced fluorescence imaging of molecular rotors in living cells, Conference on Multiphoton Microscopy in the Biomedical Sciences XI, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
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- Citations: 2
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