Imperial College London
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Professor Marina Kuimova

Faculty of Natural Sciences, Department of Chemistry

Professor of Chemical Physics
 
 
 
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Contact

 

+44 (0)20 7594 8558m.kuimova

 
 
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Location

 

207BMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kuimova:2019:2050-6120/ab481f,
author = {Kuimova, M and Kubankova, M and Chambers, J and Marciniak, S and Bond, P and Huber, R},
doi = {2050-6120/ab481f},
journal = {Methods and Applications in Fluorescence},
title = {Linker length affects photostability of protein-targeted sensor of cellular microviscosity},
url = {http://dx.doi.org/10.1088/2050-6120/ab481f},
volume = {7},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Viscosity sensitive fluorophores termed ‘molecular rotors’ represent a convenient and quantitative tool for measuring intracellular viscosity via Fluorescence Lifetime Imaging Microscopy (FLIM). We compare the FLIM performance of two BODIPY-based molecular rotors bound to HaloTag protein expressed in different subcellular locations. While both rotors are able to penetrate live cells and specifically label the desired intracellular location, we found that the rotor with a longer HaloTag protein recognition motif was significantly affected by photo-induced damage when bound to the HaloTag protein, while the other dye showed no changes upon irradiation. Molecular dynamics modelling indicates that the irradiation-induced electron transfer between the BODIPY moiety and the HaloTag protein is a plausible explanation for these photostability issues. Our results demonstrate that binding to the targeted protein may significantly alter the photophysical behaviour of a fluorescent probe and therefore its thorough characterisation in the protein bound form is essential prior to any in vitro and in cellulo applications.
AU  - Kuimova,M
AU  - Kubankova,M
AU  - Chambers,J
AU  - Marciniak,S
AU  - Bond,P
AU  - Huber,R
DO  - 2050-6120/ab481f
PY  - 2019///
SN  - 2050-6120
TI  - Linker length affects photostability of protein-targeted sensor of cellular microviscosity
T2  - Methods and Applications in Fluorescence
UR  - http://dx.doi.org/10.1088/2050-6120/ab481f
UR  - http://hdl.handle.net/10044/1/73663
VL  - 7
ER  -
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