Imperial College London
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Professor Marina Kuimova

Faculty of Natural Sciences, Department of Chemistry

Professor of Chemical Physics
 
 
 
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Contact

 

+44 (0)20 7594 8558m.kuimova

 
 
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Location

 

207BMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kuimova:2020:10.1038/s41598-020-70972-5,
author = {Kuimova, M and Kashirina, A and Lopez, Duarte I and Kubankova, M and Gulin, A and Dudenkova, V and Rodimova, S and Torgomyan, H and Zagaynova, E and Meleshina, A},
doi = {10.1038/s41598-020-70972-5},
journal = {Scientific Reports},
title = {Monitoring membrane viscosity in differentiating stem cells using BODIPY-based molecular rotors and FLIM},
url = {http://dx.doi.org/10.1038/s41598-020-70972-5},
volume = {10},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Membrane fluidity plays an important role in many cell functions such as cell adhesion, and migration. In stem cell lines membrane fluidity may play a role in differentiation. Here we report the use of viscosity-sensitive fluorophores based on a BODIPY core, termed “molecular rotors”, in combination with Fluorescence Lifetime Imaging Microscopy, for monitoring of plasma membrane viscosity changes in mesenchymal stem cells (MSCs) during osteogenic and chondrogenic differentiation. In order to correlate the viscosity values with membrane lipid composition, the detailed analysis of the corresponding membrane lipid composition of differentiated cells was performed by time-of-flight secondary ion mass spectrometry. Our results directly demonstrate for the first time that differentiation of MSCs results in distinct membrane viscosities, that reflect the change in lipidome of the cells following differentiation.
AU  - Kuimova,M
AU  - Kashirina,A
AU  - Lopez,Duarte I
AU  - Kubankova,M
AU  - Gulin,A
AU  - Dudenkova,V
AU  - Rodimova,S
AU  - Torgomyan,H
AU  - Zagaynova,E
AU  - Meleshina,A
DO  - 10.1038/s41598-020-70972-5
PY  - 2020///
SN  - 2045-2322
TI  - Monitoring membrane viscosity in differentiating stem cells using BODIPY-based molecular rotors and FLIM
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-020-70972-5
UR  - http://hdl.handle.net/10044/1/82107
VL  - 10
ER  -
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