Imperial College London
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Professor Marina Kuimova

Faculty of Natural Sciences, Department of Chemistry

Professor of Chemical Physics
 
 
 
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Contact

 

+44 (0)20 7594 8558m.kuimova

 
 
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Location

 

207BMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Vysniauskas:2021:10.1021/acssensors.0c02275,
author = {Vysniauskas, A and Cornell, B and Sherin, P and Maleckait, K and Kubankova, M and Izquierdo, MA and Vu, TT and Volkova, Y and Budynina, E and Molteni, C and Kuimova, M},
doi = {10.1021/acssensors.0c02275},
journal = {ACS Sensors},
pages = {2158--2167},
title = {Cyclopropyl substituents transform the viscosity-sensitive BODIPY molecular rotor into a temperature sensor},
url = {http://dx.doi.org/10.1021/acssensors.0c02275},
volume = {6},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A quantitative fluorescent probe that responds to changes in temperature is highly desirable for studies of biological environments, particularly in cellulo. Here, we report new cell-permeable fluorescence probes based on the BODIPY moiety that respond to environmental temperature. The new probes were developed on the basis of a well-established BODIPY-based viscosity probe by functionalization with cyclopropyl substituents at α and β positions of the BODIPY core. In contrast to the parent BODIPY fluorophore, α-cyclopropyl-substituted fluorophore displays temperature-dependent time-resolved fluorescence decays showing greatly diminished viscosity dependence, making it an attractive sensor to be used with fluorescence lifetime imaging microscopy (FLIM). We performed theoretical calculations that help rationalize the effect of the cyclopropyl substituents on the photophysical behavior of the new BODIPYs. In summary, we designed an attractive new quantitative FLIM-based temperature probe that can be used for temperature sensing in live cells.
AU  - Vysniauskas,A
AU  - Cornell,B
AU  - Sherin,P
AU  - Maleckait,K
AU  - Kubankova,M
AU  - Izquierdo,MA
AU  - Vu,TT
AU  - Volkova,Y
AU  - Budynina,E
AU  - Molteni,C
AU  - Kuimova,M
DO  - 10.1021/acssensors.0c02275
EP  - 2167
PY  - 2021///
SN  - 2379-3694
SP  - 2158
TI  - Cyclopropyl substituents transform the viscosity-sensitive BODIPY molecular rotor into a temperature sensor
T2  - ACS Sensors
UR  - http://dx.doi.org/10.1021/acssensors.0c02275
UR  - http://hdl.handle.net/10044/1/89884
VL  - 6
ER  -
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