Imperial College London
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Professor Marina Kuimova

Faculty of Natural Sciences, Department of Chemistry

Professor of Chemical Physics
 
 
 
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Contact

 

+44 (0)20 7594 8558m.kuimova

 
 
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Location

 

207BMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sherin:2021:10.1016/j.jphotobiol.2021.112346,
author = {Sherin, PS and Vysniauskas, A and Lopez-Duarte, I and Ogilby, PR and Kuimova, MK},
doi = {10.1016/j.jphotobiol.2021.112346},
journal = {Journal of Photochemistry and Photobiology, B: Biology},
pages = {1--10},
title = {Visualising UV-A light-induced damage to plasma membranes of eye lens},
url = {http://dx.doi.org/10.1016/j.jphotobiol.2021.112346},
volume = {225},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - An eye lens is constantly exposed to the solar UV radiation, which is considered the most important external source of age-related changes to eye lens constituents. The accumulation of modifications of proteins and lipids with age can eventually lead to the development of progressive lens opacifications, such as cataracts. Though the impact of solar UV radiation on the structure and function of proteins is actively studied, little is known about the effect of photodamage on plasma membranes of lens cells. In this work we exploit Fluorescence Lifetime Imaging Microscopy (FLIM), together with viscosity-sensitive fluorophores termed molecular rotors, to study the changes in viscosity of plasma membranes of porcine eye lens resulting from two different types of photodamage: Type I (electron transfer) and Type II (singlet oxygen) reactions. We demonstrate that these two types of photodamage result in clearly distinct changes in viscosity – a decrease in the case of Type I damage and an increase in the case of Type II processes. Finally, to simulate age-related changes that occur in vivo, we expose an intact eye lens to UV-A light under anaerobic conditions. The observed decrease in viscosity within plasma membranes is consistent with the ability of eye lens constituents to sensitize Type I photodamage under natural irradiation conditions. These changes are likely to alter the transport of metabolites and predispose the whole tissue to the development of pathological processes such as cataracts.
AU  - Sherin,PS
AU  - Vysniauskas,A
AU  - Lopez-Duarte,I
AU  - Ogilby,PR
AU  - Kuimova,MK
DO  - 10.1016/j.jphotobiol.2021.112346
EP  - 10
PY  - 2021///
SN  - 1011-1344
SP  - 1
TI  - Visualising UV-A light-induced damage to plasma membranes of eye lens
T2  - Journal of Photochemistry and Photobiology, B: Biology
UR  - http://dx.doi.org/10.1016/j.jphotobiol.2021.112346
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000715763300005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.sciencedirect.com/science/article/pii/S1011134421002256?via%3Dihub
UR  - http://hdl.handle.net/10044/1/94484
VL  - 225
ER  -
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