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@article{Mitra:2021:10.1186/s13073-021-00977-w,
author = {Mitra, A and MacIntyre, DA and Paraskevaidi, M and Moscicki, A-B and Mahajan, V and Smith, A and Lee, YS and Lyons, D and Paraskevaidis, E and Marchesi, JR and Bennett, PR and Kyrgiou, M},
doi = {10.1186/s13073-021-00977-w},
journal = {Genome Medicine: medicine in the post-genomic era},
title = {The vaginal microbiota and innate immunity after local excisional treatment for cervical intraepithelial neoplasia},
url = {http://dx.doi.org/10.1186/s13073-021-00977-w},
volume = {13},
year = {2021}
}

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TY  - JOUR
AB  - Background:Vaginal microbiota (VMB) composition is altered in women with cervical intra-epithelial neoplasia (CIN) compared to healthy controls and is associated with disease progression. However, the impact of CIN excision on the VMB and innate immunity is not known. This observational study aims to explore the impact of CIN excision on the VMB, antimicrobial peptides (AMP) and proinflammatory cytokines.Methods:We sampled 103 non-pregnant, premenopausal women at the time of excisional treatment for CIN and at their 6-month follow-up visit. A further 39 untreated controls with normal cytology were also sampled. We used metataxonomics to group vaginal swab samples into community state types (CSTs) and ELISA to quantify cytokine and AMP levels in matched vaginal secretions. Analyses were performed to compare the bacterial composition and immune analyte levels before and after CIN excision and in healthy controls.Results:Women with CIN had significantly higher rates of Lactobacillus species depletion pre-treatment compared to healthy controls (CST IV 21/103, 20% vs 1/39, 3%, p = 0.0081). Excision did not change the VMB composition, with CST IV remaining significantly more prevalent after excision compared to untreated, healthy controls (CST IV 19/103, 20% vs 1/39, 3%, p = 0.0142). Prevotella bivia and Sneathia amnii were significantly higher in samples before treatment compared to untreated controls, and Prevotella bivia remained significantly higher amongst the treated, with less Lactobacillus crispatus compared to untreated controls. IL-1β and IL-8 remained significantly elevated pre- (p < 0.0001 and p = 0.0014, respectively) and post-treatment (p < 0.0001 and p = 0.0035, respectively) compared to untreated controls. Levels of human beta-defensin-1 and secretory leukocyte protease inhibitor were both significantly reduced following CIN excision (p < 0.0001); however, their levels remained lower than controls post-treatment.Conclusions:Women with CIN hav
AU  - Mitra,A
AU  - MacIntyre,DA
AU  - Paraskevaidi,M
AU  - Moscicki,A-B
AU  - Mahajan,V
AU  - Smith,A
AU  - Lee,YS
AU  - Lyons,D
AU  - Paraskevaidis,E
AU  - Marchesi,JR
AU  - Bennett,PR
AU  - Kyrgiou,M
DO  - 10.1186/s13073-021-00977-w
PY  - 2021///
SN  - 1756-994X
TI  - The vaginal microbiota and innate immunity after local excisional treatment for cervical intraepithelial neoplasia
T2  - Genome Medicine: medicine in the post-genomic era
UR  - http://dx.doi.org/10.1186/s13073-021-00977-w
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000714577400001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/92662
VL  - 13
ER  -

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