Imperial College London
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ProfessorMaudLemoine

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor and Honorary Consultant in Hepatology
 
 
 
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Contact

 

+44 (0)20 3312 5212m.lemoine

 
 
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Location

 

Queen Elizabeth the Queen Mother Wing (QEQM)St Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Mohamed:2020:10.1111/liv.14315,
author = {Mohamed, Z and Mbwambo, J and Rwegasha, J and Mgina, N and Doulla, B and mwakale, P and Tuaillon, E and Chevaliez, S and Shimakawa, Y and Taylor-Robinson, S and Thursz, M and brown, A and Lemoine, M},
doi = {10.1111/liv.14315},
journal = {Liver International},
pages = {514--521},
title = {In-field evaluation of Xpert® HCV viral load fingerstick assay in people who inject drugs in Tanzania},
url = {http://dx.doi.org/10.1111/liv.14315},
volume = {40},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundAlthough novel hepatitis C (HCV) RNA point-of-care technology has the potential to enhance diagnosis in resource-limited settings, very little real-world validation of their utility exists. We evaluate the performance of HCV RNA quantification using the Xpert® HCV Viral Load Fingerstick assay (Xpert® HCV VL Fingerstick assay) as compared to the WHO pre-qualified plasma Xpert® HCV viral load assay among people who inject drugs (PWID) attending an opioid agonist therapy (OAT) clinic in Dar-es-Salaam, Tanzania. MethodsBetween December 2018 and February 2019 consecutive HCV seropositive PWID attending the OAT clinic provided paired venous and finger-stick samples for HCV RNA quantification. These were processed on-site using the GeneXpert® platform located at the Central tuberculosis reference laboratory. ResultsA total of 208 out of 220 anti-HCV positive participants recruited (94.5%) had a valid Xpert® HCV VL result available; 126 (61%; (95% CI 53.8-67.0) had detectable and quantifiable HCV RNA. 188 (85%) had paired plasma and finger-stick whole blood samples; the sensitivity and specificity for the quantification of HCV RNA levels were 99.1% and 98.7% respectively. There was an excellent correlation (R2=0.95) and concordance (mean difference 0.13 IU/mL, (95% CI -0.9 to 0.16 IU/mL) in HCV RNA levels between plasma samples and finger-stick samples.ConclusionThis study found excellent performance of the Xpert® HCV VL Fingerstick assay for HCV RNA detection and quantification in an African-field setting. Its clinical utility represents an important watershed in overcoming existing challenges to HCV diagnosis, which should play a crucial role in HCV elimination in Africa.
AU  - Mohamed,Z
AU  - Mbwambo,J
AU  - Rwegasha,J
AU  - Mgina,N
AU  - Doulla,B
AU  - mwakale,P
AU  - Tuaillon,E
AU  - Chevaliez,S
AU  - Shimakawa,Y
AU  - Taylor-Robinson,S
AU  - Thursz,M
AU  - brown,A
AU  - Lemoine,M
DO  - 10.1111/liv.14315
EP  - 521
PY  - 2020///
SN  - 1478-3223
SP  - 514
TI  - In-field evaluation of Xpert® HCV viral load fingerstick assay in people who inject drugs in Tanzania
T2  - Liver International
UR  - http://dx.doi.org/10.1111/liv.14315
UR  - https://onlinelibrary.wiley.com/doi/full/10.1111/liv.14315
UR  - http://hdl.handle.net/10044/1/75260
VL  - 40
ER  -
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