Imperial College London
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ProfessorMichaelLevin

Faculty of Medicine, Department of Infectious Disease

Chair in Paediatrics & International Child Health
 
 
 
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Contact

 

+44 (0)20 7594 3760m.levin Website

 
 
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Location

 

233Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Broderick:2022:10.1101/2022.05.26.22274729,
author = {Broderick, C and Calle, IR and Gómez, Carballa A and Gómez-Rial, J and Li, HK and Mehta, R and Jackson, H and Salas, A and Martinón-Torres, F and Sriskandan, S and Levin, M and Kaforou, M},
doi = {10.1101/2022.05.26.22274729},
title = {Pseudotemporal whole blood transcriptional profiling of COVID-19 patients stratified by clinical severity reveals differences in immune responses and possible role of monoamine oxidase B},
url = {http://dx.doi.org/10.1101/2022.05.26.22274729},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Abstract</jats:title><jats:p>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is associated with highly variable clinical outcomes. Studying the temporal dynamics of host whole blood gene expression during SARS-CoV-2 infection can elucidate the biological processes that underlie these diverse clinical phenotypes. We employed a novel pseudotemporal approach using MaSigPro to model and compare the trajectories of whole blood transcriptomic responses in patients with mild, moderate and severe COVID-19 disease. We identified 5,267 genes significantly differentially expressed (SDE) over pseudotime and between severity groups and clustered these genes together based on pseudotemporal trends. Pathway analysis of these gene clusters revealed upregulation of multiple immune, coagulation, platelet and senescence pathways with increasing disease severity and downregulation of T cell, transcriptional and cellular metabolic pathways. The gene clusters exhibited differing pseudotemporal trends. Monoamine oxidase B was the top SDE gene, upregulated in severe>moderate>mild COVID-19 disease. This work provides new insights into the diversity of the host response to SARS-CoV-2 and disease severity and highlights the utility of pseudotemporal approaches in studying evolving immune responses to infectious diseases.</jats:p>
AU  - Broderick,C
AU  - Calle,IR
AU  - Gómez,Carballa A
AU  - Gómez-Rial,J
AU  - Li,HK
AU  - Mehta,R
AU  - Jackson,H
AU  - Salas,A
AU  - Martinón-Torres,F
AU  - Sriskandan,S
AU  - Levin,M
AU  - Kaforou,M
DO  - 10.1101/2022.05.26.22274729
PY  - 2022///
TI  - Pseudotemporal whole blood transcriptional profiling of COVID-19 patients stratified by clinical severity reveals differences in immune responses and possible role of monoamine oxidase B
UR  - http://dx.doi.org/10.1101/2022.05.26.22274729
ER  -
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