Imperial College London
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ProfessorMichaelLevin

Faculty of Medicine, Department of Infectious Disease

Chair in Paediatrics & International Child Health
 
 
 
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Contact

 

+44 (0)20 7594 3760m.levin Website

 
 
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Location

 

233Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bachanová:2022:10.1038/s41598-022-20409-y,
author = {Bachanová, P and Cheyne, A and Broderick, C and Newton, SM and Levin, M and Kaforou, M},
doi = {10.1038/s41598-022-20409-y},
journal = {Scientific Reports},
title = {Comparative transcriptomic analysis of whole blood mycobacterial growth assays and tuberculosis patients’ blood RNA profiles},
url = {http://dx.doi.org/10.1038/s41598-022-20409-y},
volume = {12},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Abstract</jats:title><jats:p>In vitro whole blood infection models are used for elucidating the immune response to <jats:italic>Mycobacterium tuberculosis</jats:italic> (<jats:italic>Mtb</jats:italic>). They exhibit commonalities but also differences, to the in vivo blood transcriptional response during natural human <jats:italic>Mtb</jats:italic> disease. Here, we present a description of concordant and discordant components of the immune response in blood, quantified through transcriptional profiling in an in vitro whole blood infection model compared to whole blood from patients with tuberculosis disease. We identified concordantly and discordantly expressed gene modules and performed in silico cell deconvolution. A high degree of concordance of gene expression between both adult and paediatric in vivo<jats:italic>–</jats:italic>in vitro tuberculosis infection was identified. Concordance in paediatric in vivo vs in vitro comparison is largely characterised by immune suppression, while in adults the comparison is marked by concordant immune activation, particularly that of inflammation, chemokine, and interferon signalling. Discordance between in vitro and in vivo increases over time and is driven by T-cell regulation and monocyte-related gene expression, likely due to apoptotic depletion of monocytes and increasing relative fraction of longer-lived cell types, such as T and B cells. Our approach facilitates a more informed use of the whole blood in vitro model, while also accounting for its limitations.</jats:p>
AU  - Bachanová,P
AU  - Cheyne,A
AU  - Broderick,C
AU  - Newton,SM
AU  - Levin,M
AU  - Kaforou,M
DO  - 10.1038/s41598-022-20409-y
PY  - 2022///
TI  - Comparative transcriptomic analysis of whole blood mycobacterial growth assays and tuberculosis patients’ blood RNA profiles
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-022-20409-y
VL  - 12
ER  -
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