Imperial College London
Alternate

DrMarieLoh

Faculty of Medicine, School of Public Health

Honorary Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 2885m.loh

 
 
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Location

 

172Praed StreetSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Subramaniam:2014:10.1002/mc.21951,
author = {Subramaniam, MM and Loh, M and Chan, JY and Liem, N and Lim, PL and Peng, YW and Lim, XY and Yeoh, KG and Iacopetta, B and Soong, R and Salto-Tellez, M},
doi = {10.1002/mc.21951},
journal = {Mol Carcinog},
pages = {98--108},
title = {The topography of DNA methylation in the non-neoplastic colonic mucosa surrounding colorectal cancers.},
url = {http://dx.doi.org/10.1002/mc.21951},
volume = {53},
year = {2014}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The degree of gene hypermethylation in non-neoplastic colonic mucosa (NNCM) is a potentially important event in the development of colorectal cancer (CRC), particularly for the subgroup with a CpG island methylator phenotype (CIMP). In this study, we aimed to use an unbiased and high-throughput approach to evaluate the topography of DNA methylation in the non-neoplastic colonic mucosa (NNCM) surrounding colorectal cancer (CRC). A total of 61 tissue samples comprising 53 NNCM and 8 tumor samples were obtained from hemicolectomy specimens of two CRC patients (Cases 1 and 2). NNCM was stripped from the underlying colonic wall and samples taken at varying distances from the tumor. The level of DNA methylation in NNCM and tumor tissues was assessed at 1,505 CpG sites in 807 cancer-related genes using Illumina GoldenGate® methylation arrays. Case 1 tumor showed significantly higher levels of methylation compared to surrounding NNCM samples (P < 0.001). The average level of methylation in NNCM decreased with increasing distance from the tumor (r = -0.418; P = 0.017), however this was not continuous and "patches" with higher levels of methylation were observed. Case 2 tumor was less methylated than Case 1 tumor (average β-value 0.181 vs. 0.415) and no significant difference in the level of methylation was observed in comparison to the surrounding NNCM. No evidence was found for a diminishing gradient of methylation in the NNCM surrounding CRC with a high level of methylation. Further work is required to determine whether CIMP+ CRC develop from within "patches" of NCCM that display high levels of methylation.
AU  - Subramaniam,MM
AU  - Loh,M
AU  - Chan,JY
AU  - Liem,N
AU  - Lim,PL
AU  - Peng,YW
AU  - Lim,XY
AU  - Yeoh,KG
AU  - Iacopetta,B
AU  - Soong,R
AU  - Salto-Tellez,M
DO  - 10.1002/mc.21951
EP  - 108
PY  - 2014///
SP  - 98
TI  - The topography of DNA methylation in the non-neoplastic colonic mucosa surrounding colorectal cancers.
T2  - Mol Carcinog
UR  - http://dx.doi.org/10.1002/mc.21951
UR  - https://www.ncbi.nlm.nih.gov/pubmed/22911899
VL  - 53
ER  -
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